环境工程学报2017，Vol.11Issue(11)：6177-6183,7.DOI:10.12030/j.cjee.201608158
生物炭基固定化菌剂对石油类污染物的高效降解
Efficient degradation of petroleum pollutant by immobilized bacteria based on biochar material
摘要
Abstract
In this study,we selected biochar as the carrier medium to prepare immobilized bacteria by adsorption.The effect of different biochar structures on immobilization was analyzed.The SEM and EDS analyses showed that most microorganisms were immobilized on the surface of the biochar.The carbon,nitrogen,and phosphorus content of biochar can provide nutrients to the degrading bacteria.The porous structure of biochar can promote the degradation of petroleum pollutants.The results indicated that the oil-removal rates of F-3 strain,R-7 strain,and their mixed strain were significantly increased by 41.7％ and 29.5％,52.5％ and 42.8％,63.8％ and 53.2％ after being immobilized by corncob-biochar and straw-biochar,respectively.Oil-removal rate of the corncob-biochar-immobilized bacteria was 10.6％ higher than that of the straw-biochar-immobilized bacteria.The surface of the corncob-biochar was rougher than that of the straw-biochar.The number of immobilized microorganisms in corncob-biochar was 4.2 × 1010 cfu · g-1 and the immobilization rate was 71.2％.The number of immobilized microorganisms in straw-biochar was 2.5 × 1010 cfu · g-1 and the immobilization rate was 57.6％.The optimal preparation conditions of immobilized bacteria were as follows:the corncob-biochar was pyrolyzed at 500 ℃ for 3 h as the carrier,the microbial inoculation level was 10％,the biochar dosage was 10 g · L-1,and the immobilization temperature,rotation speed,and time were 35 ℃,130 r · min-1 and 18 h,respectively.关键词
生物炭/固定化菌剂/除油率/结构/固定化条件Key words
biochar/immobilized bacteria/oil-removal rate/structure/immobilization condition分类
资源环境引用本文复制引用
任宏洋,马伶俐,王兵,袁增,李珍珍,丁梦娇..生物炭基固定化菌剂对石油类污染物的高效降解[J].环境工程学报,2017,11(11):6177-6183,7.基金项目
四川省科技支撑计划项目(15ZC1322) （15ZC1322）
中国石油天然气集团公司科学研究与技术开发项目(2016D-5006-08) （2016D-5006-08）
