河南中医2017，Vol.37Issue(11)：1928-1930,3.DOI:10.16367/j.issn.1003-5028.2017.11.0666
糖痹康对糖尿病周围神经病变大鼠氧化应激的影响
Influence of Tangbikang on Oxidative Stress in Rats with Diabetic Peripheral Neuropathy
摘要
Abstract
Objective:To observe the influenee of Tangbikang on the levels of SOD,NO and NGF in rats with diabetic peripheral neuropathy (DPN) and explore the antioxidant effect on DPN rats.Methods:Ten rats among the 80 healthy SD rats were selected as normal control group fed with normal diet and the rest of the rats were fed with high-fat diet.After 8 weeks,STZ models were established.STZ were made into 0.45％ solution by sterile sodium citrate buffer solution (pH 4.5,4℃).Rats were given left intraperitoneal injection with standard dosage of45mg/kg.After 72h,the tail-tip blood glucose was detected with glucose meter.If the continuous blood glucose level ≥ 16.7mmol/L and kept for 3d,the rats were successful established models.The rats were randomized into 5 groups according to the body mass,10 cases in each group:Model group,Mecobalamin group (western medicine group),Tangbikang low dosage group,Tangbikang medium dosage group and Tangbikang high dosage group.Tangbikang low dose group were given administration of 4.175mg/kg/d (5 times of adult dosage);Tangbikang medium dosage group were given administration of 8.35mg/kg/d (10 times of adult dosage);Tangbikang high dosage group were given administration of 16.7 mg/(kg · d)-1 (20 times of adult dosage);these three groups were intragastric administration replaced by Tangbikang of 3 mL;Meeobalamin group were given administration of 1.97 mg/(kg · d)-1(10 times of adult dosage) and intragastrie administration of suspension made by distilled water (3 mL);model group and normal group were given intragastric administration of distilled water of equal quantity.The enzyme activities of SOD,NO and NGF in the serum of rats were detected by ELISA.Results:Compared with model group,Tangbikang groups of low,medium and high dosage improved significantly;the effect in Tangbikang group of high dosage was superior to that in control group.Tangbikang groups of low,medium and high dosage can obviously increase the level of NGF.Conclusion:The Chinese medicine Tangbikang can improve the levels of SOD,NO and NGF,inhibit the excessive oxidative stress level,prevent and delay the occurrence and development of DPN.关键词
糖尿病周围神经/糖痹康/氧化应激/SOD/NO/NGF/大鼠Key words
diabetic peripheral nerves/Tangbikang/oxidative stress/SOD/NO/NGF/rats分类
医药卫生引用本文复制引用
穆晓红,刘铜华,秦灵灵,孙文,吴丽丽,李伟笠..糖痹康对糖尿病周围神经病变大鼠氧化应激的影响[J].河南中医,2017,37(11):1928-1930,3.基金项目
国家自然科学基金(编号:81202970) （编号:81202970）
