湖南中医药大学学报2017，Vol.37Issue(11)：1218-1221,4.DOI:10.3969/j.issn.1674-070X.2017.11.012
羟基红花黄色素A对雌激素效应相关蛋白表达的影响
Influence of Hydroxysafflor Yellow A on Related Protein Expression of Estrogenic Effects
摘要
Abstract
Objective To explore the effect of hydroxylsafflor yellow A (HSYA) on related protein of estrogenic effect. Methods The positive cells T47D and ER, and negative cells SK-BR-3 of estrogen receptor (ER) were cultured, and 1×10-7 mol/L× 10-9 mol/L of HSYA act on this two cells., negative cells SK-BR-3. The expression of protein ERα, ERβ, extracellular regu-lated protein kinases 1/2 (ERK1/2), p-ERK1/2, Progesterone Receptor (PR), P27 was detected by Western Blot. Results Com-pared with the blank control group, the high concentration HSYA promoted the expression of Erα, ERβ, ERK1/2, p-ERK1/2, PR, while inhibite the expression of P27 in T47D cells (P<0.01). HSYA showed significant inhibitory effects on ERK1/2, while had no obvious effects on p-ERK1/2 in SK-BR-3 cells (P>0.05). Conclusion HSYA has different influnces on related protein of estrogenic effect in the cells, which possiblly through regulating the expression of ER and activating the ERK sig-naling pathway.关键词
羟基红花黄色素A/WesternBlot/T47D细胞/SK-BR-3细胞Key words
hydroxysafflor yellow A/Western Blot/T47D cell/SK-BR-3 cell分类
医药卫生引用本文复制引用
陈梦,赵丕文,孙丽萍,武虹波,赵笛..羟基红花黄色素A对雌激素效应相关蛋白表达的影响[J].湖南中医药大学学报,2017,37(11):1218-1221,4.基金项目
国家自然科学青年基金(81673764) （81673764）
北京中医药大学自主科研课题(2017-JYB-JS-001). （2017-JYB-JS-001）
