四川大学学报（医学版）2017，Vol.48Issue(6)：819-823,5.
甲基化调控microRNA表达影响胰腺癌细胞增殖、迁移、侵袭的研究
The Study of Methylated Regulation MicroRNA in Pancreatic Carcinoma Cell and Its Effect on Cell Proliferation,Migration and Invasion
胡开锋 1杨秋韵 1董婧颖 1杨晓龙 1彭恒 1陈利弘 1孙艺 1马兰枝 1刘戟1
作者信息
- 1. 四川大学华西基础医学与法医学院生物化学与分子生物学教研室 成都610041
- 折叠
摘要
Abstract
Objective To study the epigenetic regulation of pancreatic carcinoma related microRNA (miR34a,miR34b,miR148a and miR203a) expression by gene promoter methylation,and its effect on the proliferation,migration and invasion of pancreatic carcinoma cells.Methods The pancreatic carcinoma cells were divided into two groups:control group and treatment group.Control group was treated with 0 μmol/L DNA methyltransferase inhibitor 5-Aza-CdR and treatment group was treated with 60 μmol/L 5-Aza-CdR.The methylation status of microRNA gene promoter regions was detected by MSP (methylation-specific PCR).The microRNAs' expression levels were evaluated by real-time PCR.The CCK-8 assay,wound healing assay and Transwell assay were employed to study the proliferation,migration and invasion of pancreatic carcinoma cells,respectively.Results The results of MSP showed that the methylated band of the treated group was weaker than that of the untreated group and the unmethylated band of the treated group was stronger than that of the untreated group.Real-time PCR results showed that the relative expression levels of microRNAs in the treatment group were higher than those in the control group (P＜0.05).The CCK-8 assay showed that inhibition rate of the treatment group showed dose-dependent effect with the increase of drug concentration.Wound healing assay showed that the wound healing rate of Treatment group was lower than that of untreated group (P＜0.01).The results of transwell assay showed that the number of migrated cells in the treated group was less than that in the untreated group (P＜0.01).Conclusion Decreased methylation levels in microRNA promoter region caused by 5-Aza-CdR treatment increased the expression of miR34a,miR34b,miR148a and miR203a,leading to inhibition of the proliferation,migration and invasion of pancreatic carcinoma cells.关键词
microRNA/甲基化/5-Aza-CdR/胰腺癌/增殖/迁移Key words
microRNA/Methylation/5-Aza-CdR/Pancreatic carcinoma/Proliferation/Migration引用本文复制引用
胡开锋,杨秋韵,董婧颖,杨晓龙,彭恒,陈利弘,孙艺,马兰枝,刘戟..甲基化调控microRNA表达影响胰腺癌细胞增殖、迁移、侵袭的研究[J].四川大学学报（医学版）,2017,48(6):819-823,5.
