四川大学学报（医学版）2017，Vol.48Issue(6)：873-876,4.
长链非编码RNA-Paupar在局麻药致神经毒性过程中的作用研究
The Role of Long Chain Non-coding RNA-Paupar in the Process of Bupivacaine Induced Neurotoxicity
摘要
Abstract
Objective To observe the expression mode and modulation effects of lncRNA-Paupar in the process of bupivacaine induced neurotoxicity.Methods Dorsal root ganglion (DRG) neurons were cultured and neurotoxicity model was produced on it.And the expression of lncRNA-Paupar was evaluated by qRT-PCR.Lnc-Paupar specific siRNA P and random control group siRNA-C were constructed,200 nmol/L siRNA-P,siRNA-C were transfected into ganglion cells and the apoptosis rate of transfected cells were observed by TUNEL method.The JNK and phosphorylated JNK (p-JNK) protein expression were observed with Western blot,respectively.Results LncRNA-Paupar was significantly up-regulated during the process of bupivacaine induced neurotoxicity and siRNA mediated lncRNA-Paupar down regulation protected DRG neurons cells from apoptosis.Both JNK and p-JNK protein were reduced in siRNA transfected cells exemplified by Western blot.Conclusion LncRNA-Paupar could induced neurotoxicity through JNK pathway.关键词
长链非编码RNA/神经毒性/布比卡因/凋亡Key words
LncRNA/Neurotoxicity/Bupivacaine/Apoptosis引用本文复制引用
张利亮,祁莉娜,姚泽宇..长链非编码RNA-Paupar在局麻药致神经毒性过程中的作用研究[J].四川大学学报（医学版）,2017,48(6):873-876,4.基金项目
青海省卫计委基金(No.2016wyzd-11)资助 （No.2016wyzd-11）
