四川大学学报（医学版）2017，Vol.48Issue(6)：880-885,6.
miRNA成熟相关基因DICER、DROSHA、RAN基因多态性与不明原因复发流产的相关性研究
The Association Between the Polymorphisms of miRNA Biogenesis Related Genes (DICER, DROSHA and RAN) and Unexplained Recurrent Spontaneous Abortion in Chinese Women
摘要
Abstract
Objective To explore the association between polymorphisms of miRNA biogenesis related gene DICER,DROSHA,RAN and unexplained recurrent spontaneous abortion (URSA) in Chinese women.Methods We recruited 217 patients with URSA (URSA group) and 390 healthy controls who were fertile women with history of more than one successful pregnancy outcome (control group) from June 2013 to December 2015 in the West China Second University Hospital of Sichuan University.The control group was recruited from regular physical examination and prepregnancy check for women of childbearing age during the same period.A case-control study was performed to analyze polymorphism of miRNA machinery genes,including DICER rs3742330,DROSHA rs10719,RAN rs14035,by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.Results The frequencies of genotypes and alleles of DICER rs3742330,DROSHA rs10719,and RAN rs14035 showed no difference between URSA patients and control group (all P ＞ 0.05).DICER rs3742330/DROSHA rs10719 GG/TC+TT combinations were synergistically associated with increased URSA risk [odds ratio (OR) =1.657,95％ confidence interval (CI) =1.006-2.731,P=0.047].Although DICER rs3742330/RAN rs14035 GG / TT+TC combinations was significantly higher in the URSA group than in the control group,there was no statistical significance (OR =1.977,95％ CI =0.956-4.087,P =0.066).However,DROSHAI0719/RAN14035 TT+TC/TT+TC had no significant correlation with URSA (OR=0.958,95 ％CI=0.679-1.353,P=0.808).Conclusion Our study demonstrated the relationship between URSA development and combined genotype of DICER rs3742330/DROSHA rs10719 GG/TC+TT.关键词
DICER/DROSHA/RAN/基因多态性/复发流产Key words
DICER/DROSHA/RAN/Gene polymorphism/Recurrent spontaneous abortion引用本文复制引用
符梅,许文明,覃太周,徐克惠..miRNA成熟相关基因DICER、DROSHA、RAN基因多态性与不明原因复发流产的相关性研究[J].四川大学学报（医学版）,2017,48(6):880-885,6.基金项目
国家重点基础研究计划(No.2012CB944903)和新世纪人才计划(No.NCET-12-0382)资助 （No.2012CB944903）
