林业工程学报2017,Vol.2Issue(6):97-102,6.DOI:10.13360/j.issn.2096-1359.2017.06.017
米根霉利用玉米芯渣同步糖化发酵联产富马酸和真菌壳聚糖
Co-production of fumaric acid and fungal chitosan from corncob by simultaneous saccharification and fermentation with Rhizopus oryzae
摘要
Abstract
The co-production of fumaric acid and fungal chitosan based on the simultaneous saccharification and fermentation (SSF) of alkali-treated corncob residues was investigated at high solid loading.Cellulase hydrolysis with a higher corncob substrate concentration resulted in a higher substrate concentration of glucose,which was benefit for the production of fumaric acid by Rhizopus oryzae.The SSF at 100 g/L substrate concentration of corncob by R.oryzae could produce 28.65 g/L of fumaric acid,which was 1.95 times than that from SSF at 50 g/L substrate concentration.After SSF,fungal chitosan was prepared from R.oryzae biomass by alkaline process.The deacetylation degree of R.oryzae chitosan was 90%.The viscosity ofR.oryzae chitosan (20 g/L) was 2 mPa·s.Weight-average molecular weight and number-average molecular weight of R.oryzae chitosan were 2 578 u and 1 532 u,respectively,measured by gel chromatography.The results of this study can provide a cost-effective approach for co-production of fumaric acid and fungal chitosan by SSF using R.oryzae from lignocellulosic materials.关键词
米根霉/同步糖化发酵/木质纤维原料/联产/富马酸/壳聚糖Key words
Rhizopus oryzae/simultaneous saccharification and fermentation/lignocellulosic materials/co-production/fumaric acid/chitosan分类
化学化工引用本文复制引用
李鑫,顾夕梅..米根霉利用玉米芯渣同步糖化发酵联产富马酸和真菌壳聚糖[J].林业工程学报,2017,2(6):97-102,6.基金项目
江苏省高校自然科学研究重大项目(14KJA220003) (14KJA220003)
江苏省重点研发计划(BF2015007) (BF2015007)
江苏高校优势学科建设工程资助项目(PAPD). (PAPD)