南京农业大学学报2017，Vol.40Issue(6)：1023-1030,8.DOI:10.7685/jnau.201704025
黄瓜根际芽孢杆菌亲缘辨识特性研究
Kin discrimination between Bacillus spp.strains from cucumber rhizosphere
摘要
Abstract
[Objectives] We investigated the ability of cucumber rhizosphere bacterium Bacillus spp.to discriminate kin from nonkin in the context of swarming and 16S rRNA gene squences,then formed a direct cooperative muticellular behavior,and screen the kin strains of Bacillus amyloliquefaciens SQR9.[Methods] The Bacillus spp.strains were isolated from plates spread with diluted solution of cucumber rhizosphere soil samples after heat shock.Then the sub family classification of those Bacillus spp.strains were identified based on 16S rRNA gene sequence analysis and swarming discrimination phenotype which were performed on swarming agar.We investigated the biocontrol potential of those kin strains of SQR9 through static culture biofilm assays in 24 well plate,IAA detection by salkowski reagent and the production of phytase.[Results] 118 Bacillus spp.strains were isolated through primary screening from cucumber rhizosphere,and 21 Bacillus spp.strains were collected after secondary screening.Three different phenotypes(boundary,intermediate and merging)were detected from Bacillus spp.strains swarms.Out of 27 merging swarm combinations,25 combinations had 100％ identical 16S rRNA gene,while the other two showed lower nucleotide identity.In contrast,only 10％ of strain pairs that formed clear boundary lines (15 combinations out of 142)had higher than 99％ identical 16S rRNA gene.In addition,combinations showed an intermediate lines shared a genetic identity ranging from 99.5％ to 100％.Three kin strains of SQR9(P2,W-4 and N-2)showed similar abilities in biofilm formation,IAA production and phytase activities when compared with SQR9.[Conclusions]Present study preliminarily proved that swarm interaction phenotype correlates with 16S rRNA,and three kin strains of SQR9 (P2,W-4 and N-2)are potential plant growth-promoting rhizobacteria.关键词
亲缘辨识/群集运动/芽孢杆菌/根际促生细菌Key words
kin discrimination/swarming/Bacillus spp./plant growth-promoting rhizobacteria分类
农业科技引用本文复制引用
徐志辉,黄蓉,张钰婷,冯元韬,张馨玉,仇美华..黄瓜根际芽孢杆菌亲缘辨识特性研究[J].南京农业大学学报,2017,40(6):1023-1030,8.基金项目
国家自然科学基金青年基金项目(31501833) （31501833）
江苏省自然科学基因青年基金项目(BK20150658) （BK20150658）
江苏高校品牌专业建设工程资助项目(PPZY2015A061) （PPZY2015A061）
