日用化学工业（中英文）2017，Vol.47Issue(11)：633-636,650,5.DOI:10.13218/j.cnki.csdc.2017.11.007
3D皮肤模型的构建及其在化妆品原料活性物功效检测中的应用研究
Reconstruction of 3D skin model and its application in evaluation the efficacy of cosmetic raw materials to be used as active ingredients
宋肖洁 1周春霞 1吴越1
作者信息
- 1. 伽蓝(集团)股份有限公司研发中心,上海 200233
- 折叠
摘要
Abstract
Via the use of Mimedisc?as a scaffold,the normal human dermal fibroblasts were first seeding into the scaffold to form a dermal equivalent after 3 weeks;and then seeding normal human epidermal keratinocytes on it,followed by immersion and air-liquid inter-phase culture stage,to finally form a 3D sponge matrix skin model which has a very similar structure of normal human skin(NHS).The results of Masson staining,immuno-histochemical(IHC)and immuno -fluorescence(IF)staining show that this skin model displays a very similar structure and key protein expression of NHS.Meanwhile,this model was used as a tool to investigate the effect of Himalaya cedar extract on Dermal-Epidermal Junction(DEJ)in vitro.Results show that the Himalayan cedar extract can significantly increase the thickness of the epidermis,thus increase the barrier capacity of the skin to resist external environment.Further,the Himalaya cedar extract can promote the expression of Laminin 5 and Collagen VII of DEJ. The expression of the Laminin 5 and Collagen VII increases 20% and 47%respectively.So the Himalaya cedar extract displays the potential to be applied in development of anti-aging cosmetics.关键词
化妆品原料/雪松/3D皮肤模型/真表皮连接处/抗衰老Key words
cosmetic raw materials/cedar/3D skin model/Dermal-Epidermal Junction/anti-aging分类
化学化工引用本文复制引用
宋肖洁,周春霞,吴越..3D皮肤模型的构建及其在化妆品原料活性物功效检测中的应用研究[J].日用化学工业（中英文）,2017,47(11):633-636,650,5.
