四川中医2017，Vol.35Issue(10)：54-57,4.
基于TLR4/NF-κB信号通路研究益脾养肝方对大鼠肝癌前病变的影响
Effects of Compound YPYGF for Hepatic Precancerous Lesion in Rats Based on TLR4/NF-κB Signaling Pathway
摘要
Abstract
Objective:By the method of dietltylmitrosainine (DENA)-induced rat liver precancerous lesion model,to observe the effect of compound YPYGF on TLR4/NF-κB signaling pathway in rats.Methods:Rats were randomly divided into normal controlled group,DENA group,and two YPYGF (0.5and 1.0g/ml) treatment groups.After 16weeks of administration,rats were sacrificed to messure the changes of liver function,expression of TLR4and NF-κB in liver tissue homogenate,and liver tissues were measured by HE staining.Results:Compared with the DENA group,the levels of ALT,AST,ALP,TBIL in serum,and the levels of TLR4and NF-κB in liver tissue homogenate in YPYGF groups were significantly lower,the high-dose group was better (P＜0.01).Liver HE staining pathological sections showed,compound YPYGF can improve the pathological changes of rats in the process of liver precancerous lesions.Conclusion:Compound YPYGF can inhibit the progression of liver precancerous lesions in rats,the mechanism of action may be through the TLR4/NF-KB signal pathway,which inhibits inflammation.关键词
肝癌前病变/DENA/益脾养肝方/TLR4/NF-κBKey words
Liver precancerous lesion/Dietltylmitrosainine/Compound YPYGF/TLR4/NF-κB分类
医药卫生引用本文复制引用
魏海梁,李京涛,闫曙光,郭辉,鞠迪,冯飞雪,常占杰..基于TLR4/NF-κB信号通路研究益脾养肝方对大鼠肝癌前病变的影响[J].四川中医,2017,35(10):54-57,4.基金项目
国家自然科学基金(编号:81603612),陕西省科技厅(编号:2012SF2-11,编号:2014K11-02-04-06,编号:2016SF-234),陕西省中医药管理局(编号:15-JC009),北京医学奖励基金会课题(编号:YJHYXKYJJ-276) （编号:81603612）
