四川中医2017，Vol.35Issue(10)：58-61,4.
希美宁对T2DM模型大鼠血清中GLP-1、GIP含量及骨骼肌PI3KmRNA、AktmRNA表达的影响
Effect of Ximeining on the Levels of GLP-1 and GIP in Serum and the Expression of PI3KmRNA and AktmRNA in Skeletal Muscle of Type 2Diabetic Rats
摘要
Abstract
Objective:To observe the effects of Ximeining on the levels of GLP-1and GIP in serum and the expression of PI3KmRNA and AktmRNA in skeletal muscle of type 2diabetic rats.Methods:Intragastric administration of fat emulsion and two small dose injection of STZ induced type 2diabetic rat model,the rats were randomly divided into controlled group,model group,Ximeining group,metformin group,enzyme linked immunosorbent assay was used to detect the levels of GLP-1 and GIP in serum of rats in each group.The expression of PI3KmRNA and AktmRNA in skeletal muscle of rats were detected by RT-PCR method.Results:Compared with the model group,Ximeining group and metformin group can increase the content of serum GLP-1,GIP (P (0.05or P (0.01),also can significantly up regulate the expression of PI3KmRNA and AktmRNA in skeletal muscle (P (0.01).Conclusion:Ximeining can promote GLP-1 and GIP secretion to regulate metabolism,but also by the change of expression of PI3KmRNA and AktmRNA to improve the T2DM rat model of insulin resistance.关键词
希美宁/2型糖尿病模型大鼠/GLP-1/GIP/PI3KmRNA/AktmRNAKey words
Ximeining/Type 2diabetes mellitus rats/GLP-1/GIP/PI3KmRNA/AktmRNA分类
医药卫生引用本文复制引用
朱洁宜,宋仲涛,张晓燕,李显筑..希美宁对T2DM模型大鼠血清中GLP-1、GIP含量及骨骼肌PI3KmRNA、AktmRNA表达的影响[J].四川中医,2017,35(10):58-61,4.基金项目
黑龙江省中医药科研项目(编号:ZHY12-W037). （编号:ZHY12-W037）
