西北林学院学报2017，Vol.32Issue(6)：145-149,5.DOI:10.3969/j.issn.1001-7461.2017.06.22
中华散尾鬼笔培养基筛选及培养条件优化
Screening and Optimization of Culture Medium and Conditions of Lysurus mokusin
摘要
Abstract
Lysurus mokusin extract has insecticidal and antifungal activities,and it is necessary to cultivate a large number of mycelium because its mycelia grow slowly in the laboratory environment.In order to improve the growth rate of Lysurus mokusin mycelia,the culture medium and conditions were screened and optimized in this study.The culture medium was screened with the growth rate of mycelium as the evaluation index,and the suitable medium for the growth of L.mokusin mycelia was selected out.And on this basis,the carbon source,nitrogen source,inorganic salt addition,pH and culture temperature were further optimized.The best culture medium was 200 g of potato,20 g of glucose,3 g of peptone,10％ inorganic salt solution (KH2PO4 1 g,K2HPO4 1 g,MgSO4 0.5 g,CaCl2 0.05 g,KCl 0.5 g,FeSO4 0.01 g and NaNOa 0.5 g dissolved in 100 mL distilled water) and 15 g of agar.The optimum culture conditions were pH=5 and temperature of 20℃.Under the above culture conditions,the net growth rate of L.mokusin mycelia was 0.59 cm/d,which was 23.69％ higher than that of medium A.关键词
中华散尾鬼笔/培养基/培养条件/生长速率Key words
Lysurus mokusin/culture medium/culture condition/screen/optimization分类
农业科技引用本文复制引用
杨璟,林连男,王瑞琦,张国珍,张晓波,张国财..中华散尾鬼笔培养基筛选及培养条件优化[J].西北林学院学报,2017,32(6):145-149,5.基金项目
中央高校基本科研业务费专项资金项目(2572016AA03) （2572016AA03）
哈尔滨市应用技术研究与开发项目(2016RAXXJ035) （2016RAXXJ035）
黑龙江省森工总局科研项目(sgzjT2015005) （sgzjT2015005）
中央高校基本科研业务费专项资金项目(2572014AA08). （2572014AA08）
