中国组织工程研究2017，Vol.21Issue(33)：5249-5254,6.DOI:10.3969/j.issn.2095-4344.2017.33.001
盐酸四环素对大鼠骨髓间充质干细胞增殖的作用
Tetracycline hydrochloride promotes rat bone marrow mesenchymal stem cells proliferation in vitro
摘要
Abstract
BACKGROUND: Tetracycline hydrochloride is shown to promote viability and proliferation of human embryonic osteoblasts cultured in vitro, increase the production of type I collage and osteocalcin. OBJECTIVE: To study the proliferation activity of bone marrow mesenchymal stem cells (BMSCs) cultured by tetracycline hydrochloride in vitro. METHODS: Passage 3 BMSCs from Sprague-Dawley rats were cultured and divided into two groups: cells in control group were cultured in complete medium containing fetal bovine serum, and those in experimental group were cultured in complete medium containing tetracycline hydrochloride. After days 1-8 of culture, MTT and cell counting assay were used to detect cell viability and proliferation in the two groups. RESULTS AND CONCLUSION: (1) Cell counting assay: with time, the number of cells in both groups was increased, but the cell number in the experimental group was higher than that in the control group at days 4-8 of culture (P < 0.05). (2) MTT assay: With time, the number of cells in both groups was increased: incubation period was at day 1, logarithmic growth period was within days 3-6, and plateau period began at day 7. Then, the cells continued to proliferate, and the cell proliferation was higher in the experimental group than the control group at days 5-8 of culture (P < 0.05). To conclude, tetracycline hydrochloride can significantly enhance BMSCs proliferation.关键词
干细胞/骨髓干细胞/盐酸四环素/骨髓间充质干细胞/增殖/国家自然科学基金分类
医药卫生引用本文复制引用
张珏,薛斯亮,罗媛,智伟..盐酸四环素对大鼠骨髓间充质干细胞增殖的作用[J].中国组织工程研究,2017,21(33):5249-5254,6.基金项目
国家自然科学基金(31400809) （31400809）
贵州省科技厅科学技术基金项目([2012]2366 号) （[2012]2366 号）
中央高校基本科研业务费专项资金(2682015CX006) the National Natural Science Foundation of China, No. 31400809 （2682015CX006）
the Science and Technology Foundation of Guizhou Science and Technology Department, No [2012]2366 （）
the Fundamental Research Funds for the Central Universities, No. 2682015CX006 （）
