中国组织工程研究2017，Vol.21Issue(33)：5262-5267,6.DOI:10.3969/j.issn.2095-4344.2017.33.003
骨髓间充质干细胞体外分化过程中复方接骨片和壮骨片含药血清的作用
Effect of Compound Jiegu Tablets and Zhuanggu Tablets on the differentiation of bone marrow mesenchymal stem cells in vitro
邬波 1柳椰 2马旭 1刘素媛 2王译晗 1王佳媛 2谭成波 2杨政博2
作者信息
- 1. 沈阳市骨科医院关节外科,辽宁省沈阳市 110044
- 2. 辽宁省骨关节病重点实验室,辽宁省沈阳市 110044
- 折叠
摘要
Abstract
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are a kind of pluripotent stem cells in the bone marrow. Drug-containing serum treatment may be followed by a variation in the expression of genes involved in multiple signaling pathways. OBJECTIVE: To study the effect of compound Jiegu Tablets and Zhuanggu Tablets on the differentiation of BMSCs in vitro. METHODS: Sixty Sprague-Dawley rats were randomized into Jiegu group, Zhuanggu group, combined group and blank control group. Different drug-containing sera were prepared and used to culture BMSCs isolated from the rat bone marrow. RT-PCR and western blot assay were used to detect the expression of BMP-2, Runx2, VEGF and Akt at mRNA and protein levels, respectively, at 24 hours after culture.RESULTS AND CONCLUSION: Compared with the blank control group, the expression levels of Akt and VEGF mRNA and protein were significantly increased in the Jiegu group and combined group (P < 0.05 or P < 0.01), but showed no difference in the Zhuanggu group (P > 0.05). Compared with the blank control group, the expression levels of BMP-2 and Runx2 mRNA and protein were significantly increased in the Jiegu group (P < 0.05) and very significantly increased in the Zhuanggu group and combined group (P < 0.01). These findings indicate that compound Jiegu Tablets and Zhuanggu Tablets containing sera lead to synergistic effects on the BMSCs differentiation into vascular endothelial cells and osteoblasts in vitro.关键词
干细胞/骨髓干细胞/复方接骨片/壮骨片/骨髓间充质干细胞/含药血清/骨形态发生蛋白/血管内皮生长因子/体外分化通路分类
医药卫生引用本文复制引用
邬波,柳椰,马旭,刘素媛,王译晗,王佳媛,谭成波,杨政博..骨髓间充质干细胞体外分化过程中复方接骨片和壮骨片含药血清的作用[J].中国组织工程研究,2017,21(33):5262-5267,6.
