中国组织工程研究2017，Vol.21Issue(33)：5274-5279,6.DOI:10.3969/j.issn.2095-4344.2017.33.005
骨髓基质干细胞与负载血管内皮生长因子/骨形态发生蛋白2聚乳酸-羟基乙酸共聚物/明胶纳米纤维支架的生物相容性
Biocompatibility of bone marrow stromal stem cells with vascular endothelial growth factor/bone morphogenetic protein 2-poly(lactic-co-glycolic acid) copolymer/gelatin nanofibrous scaffold
摘要
Abstract
BACKGROUND: Using bone tissue engineering methods for reconstruction of bone repair is an ideal treatment for bone defects, and it is crucial to combine biological scaffolds carrying growth factors with seed cells. OBJECTIVE: To observe the biocompatibility of rat bone marrow stromal stem cells (BMSCs) seeded onto vascular endothelial growth factor (VEGF)/bone morphogenetic protein 2 (BMP-2)-poly(lactic-co-glycolic acid) (PLGA) copolymer/ gelatin nanofibrous scaffolds.METHODS: Rat BMSCs were divided into five groups: blank scaffold group, BMP-2 group, VEGF group, VEGF/BMP-2 groups (1.2:1, 0.8:1, 0.4:1). In each group, the nanofibrous scaffold was placed at the bottom of the cell culture plate, and then rat BMSCs were seeded into the culture plate. Cell adhesion was observed under scanning electron microscope; cell proliferation was detected by cell counting kit-8; and ALP, RUNX-2 and OCN expression was determined by RT-PCR. RESULTS AND CONCLUSION: Under the scanning electron microscope, the BMSCs adhered to the scaffold and further grew into the scaffold. The adherent cells grew best in the 0.4:1 group, in which the cells were tightly integrated with the scaffold to form a cell-scaffold complex. Compared with the blank scaffold group, the cell proliferation and expression of ALP, RUNX-2 and OCN were both higher in the other groups, which was highest in the 0.4:1 group. To conclude, the VEGF/BMP-2-loaded PLGA copolymer/gelatin nanofibrous scaffold can promote cell adhesion, proliferation, osteogenic differentiation of rat BMSCs because of a good cytocompatibility. Moreover, the concentration ratio of VEGF/BMP-2 is optimized at 0.4:1.关键词
干细胞/骨髓干细胞/骨髓基质干细胞/聚乳酸-羟基乙酸共聚物/明胶/纳米纤维支架/骨形态发生蛋白2/血管内皮生长因子/骨组织工程分类
医药卫生引用本文复制引用
安刚,鲁斌,张文博,蒋玉东..骨髓基质干细胞与负载血管内皮生长因子/骨形态发生蛋白2聚乳酸-羟基乙酸共聚物/明胶纳米纤维支架的生物相容性[J].中国组织工程研究,2017,21(33):5274-5279,6.基金项目
黑龙江省科研计划项目(2015001) the Science and Technology Research Plan of Heilongjiang Province, No. 2015001 （2015001）
