中国组织工程研究2017，Vol.21Issue(33)：5280-5286,7.DOI:10.3969/j.issn.2095-4344.2017.33.006
大鼠肺泡Ⅱ型上皮细胞系RLE-6TN诱导脂肪间充质干细胞体外分化
In vitro differentiation of rat adipose-derived mesenchymal stem cells induced by rat lung epithelial-T-antigen negative cell line
摘要
Abstract
BACKGROUND: Studies have shown that bone marrow mesenchymal stem cells have the potential of differentiation into alveolar epithelial cells in vitro, but so far no study has indicated that adipose-derived mesenchymal stem cells (ADSCs) can be differentiated into alveolar epithelial cells through long-term Transwell co-culture. OBJECTIVE: To observe whether rat lung epithelial-T-antigen negative cell lines (RLE-6TN) can induce rat ADSCs to differentiate into type II alveolar epithelial cells by long-term Transwell co-culture. METHODS: Three SPF health female Sprague-Dawley rats were used as donors to separate, extract, culture and identity ADSCs. The experimental group was subjected to the Transwell co-culture of ADSCs and RLE-6TN, while the control group was subjected to the culture of ADSCs alone. The morphological changes of ADSCs were observed by the inverted phase contrast microscope at 21 days after co-culture. Immunofluorescence staining using surfactant protein C (SP-C) was performed on the co-cultured ADSCs. The fluorescence staining was observed using the inverted fluorescence microscope. Integral optical density (IOD) analysis was conducted by Image pro plus 6.0 software. RESULTS AND CONCLUSION: RLE-6TN cells were identified by fluorescence staining with stable expression of SP-C protein (red fluorescence) in the experimental group, and there was no red fluorescence in the control group. After 21-day co-culture, the cell shape in the experimental group was transformed from the long spindle shape into oval or polygon shape gradually, while the cell shape in the control group remained fibroblast-like. These results show that RLE-6TN can induce ADSCs to differentiate into type II alveolar epithelial cells after a long-term (21 days) co-culture.关键词
干细胞/脂肪肝细胞/脂肪间充质干细胞/肺泡上皮细胞/RLE-6TN/共培养/Transwel/细胞分化/大鼠/国家自然科学基金分类
医药卫生引用本文复制引用
陈尚雅,崔冠群,薄存香,张玉,张恩国,杨叶,杜忠君,邵华..大鼠肺泡Ⅱ型上皮细胞系RLE-6TN诱导脂肪间充质干细胞体外分化[J].中国组织工程研究,2017,21(33):5280-5286,7.基金项目
国家自然科学基金(81470145,81602893) （81470145,81602893）
山东省自然科学基金(ZR2015YL049) （ZR2015YL049）
山东省医药卫生科技发展计划(2016WS0540) （2016WS0540）
山东省医学科学院创新工程the National Natural Science Foundation of China, No. 81470145, 81602893 （）
the Natural Science Foundation of Shandong Province, No. ZR2015YL049 （）
the Medical and Health Science and Technology Development Plan, No. 2016WS0540 （）
the Innovation Engineering of Shandong Academy of Medical Sciences （）
