中国组织工程研究2017，Vol.21Issue(33)：5287-5292,6.DOI:10.3969/j.issn.2095-4344.2017.33.007
妊娠对大鼠脂肪干细胞增殖及成肝分化潜能的影响
Pregnancy influences the proliferation and hepatic differentiation of rat adipose-derived stem cells
李俊男 1陈嘉勇 2张毅 2陆晓涛1
作者信息
- 1. 昆明医科大学第四附属医院骨与创伤外科,云南省昆明市 650000
- 2. 昆明医科大学第二附属医院急诊医学部,云南省昆明市 650000
- 折叠
摘要
Abstract
BACKGROUND: Pregnancy exhibits a significant association with stem cell activity. However, there is yet no report on the effects of pregnancy on adipose-derived stem cells.OBJECTIVE: To investigate the effect of pregnancy on the proliferation and differentiation of rat adipose-derived stemcells into hepatocyte-like cells.METHODS: Adipose-derived stem cells were isolated and cultured from normal and pregnant Sprague-Dawley rats, and identified. The cell proliferation was detected by cell counting kit-8. All harvested adipose-derived stem cells were cultured in hepatocyte-induced medium and induced to differentiate into hepatocyte-like cells. Then the expressions of hepatocyte markers were detected at 0, 7, 14, 21 days of induction by fluorescent quantitative PCR; albumin secretion and urea synthesis were detected by using ELISA method. RESULTS AND CONCLUSION: Adipose-derived stem cells were successfully isolated from normal and pregnant rats, and CD45 negative staining and CD44/CD90 positive staining were observed, which was consistent with the characteristics of stem cells. Compared to the normal cells, adipose-derived stem cells from pregnant rats showed faster proliferation as well as osteogenic, adipogenic and hepatocyte-like differentiation, with the better liver function of hepatocyte-like cells. To conclude, pregnancy can promote the proliferation and differentiation of adipose-derived stem cells, which may be related to the estrogen level.关键词
干细胞/脂肪干细胞/妊娠/细胞增殖/成肝分化分类
医药卫生引用本文复制引用
李俊男,陈嘉勇,张毅,陆晓涛..妊娠对大鼠脂肪干细胞增殖及成肝分化潜能的影响[J].中国组织工程研究,2017,21(33):5287-5292,6.
