中国组织工程研究2017，Vol.21Issue(33)：5339-5347,9.DOI:10.3969/j.issn.2095-4344.2017.33.015
诱导多能干细胞对骨关节炎软骨损伤的修复作用
Cartilage repair using induced pluripotent stem cell derived chondrocytes in osteoarthritis
摘要
Abstract
BACKGROUND: Stem cell-based therapy has been proposed for the treatment of osteoarthritis (OA) and induced pluripotent stem cells (iPSCs) are becoming a promising stem cell source as they have strong proliferation and differentiation potentials and no ethics problem. OBJECTIVE: To explore an effective method of iPSCs differentiating into chondrocytes and to study the therapeutic effect of iPSCs derived chondrocytes on osteoarthritis. METHODS: In this study, three steps were developed to induce human iPSCs to differentiate into chondrocytes which were then transplanted into rat OA models induced by monosodium iodoacetate (MIA). There were four groups in the experiment: control group with normal saline injection, model group with MIA injection, iPSCs group with iPSCs transplantation following MIA injection, and differentiated iPSCs group with transplantation of iPSCs derived chondrocytes following MIA injection. At 15 weeks after transplantation, micro-CT was used and histological analysis of the knee joint was performed. RESULTS AND CONCLUSION: Compared with the iPS group, the expression of chondrocyte specific genes and proteins (Col2A1, GAG and Sox9) were significantly increased in the differentiated iPSCs group after 6 days of embryoid formation and after 2 weeks of cell differentiation. At 15 weeks after cell transplantation, no immune responses were observed, micro-CT showed an improvement in subchondral bone integrity, and histological examinations demonstrated the production of articular cartilage matrix. iPSCs derived chondrocytes showed better effects on articular cartilage repair than the iPSCs. To conclude, iPSCs derived chondrocytes can be effective via transplantation approach for cartilage tissue regeneration in OA rats.关键词
干细胞/移植/诱导多能干细胞/软骨细胞/骨关节炎/分化/移植/谷氨酸钠碘乙酸/胶原/蛋白多糖/Sox9/国家自然科学基金分类
医药卫生引用本文复制引用
吴张松,朱红霞,罗志强,吴晓敏,林光耀,张明裕,顾洪生,朱艳霞..诱导多能干细胞对骨关节炎软骨损伤的修复作用[J].中国组织工程研究,2017,21(33):5339-5347,9.基金项目
国家自然科学基金(81301597) （81301597）
深圳新兴战略产业发展专项资金(JCYJ20130326110154156/JCYJ20150525092940984/ JCYJ20160422090807181)the National Natural Science Foundation of China, No. 81301597 （JCYJ20130326110154156/JCYJ20150525092940984/ JCYJ20160422090807181）
Shenzhen Emerging Strategic Industrial Development Special Funds, No. JCYJ20130326110154156, JCYJ20150525092940984, and CYJ20160422090807181 （）
