中国组织工程研究2017，Vol.21Issue(33)：5394-5399,6.DOI:10.3969/j.issn.2095-4344.2017.33.023
LINGO-1在脊髓神经干细胞分化过程中的表达特征及效应
The expression and effect of LINGO-1 during the differentiation of spinal cord derived neural stem cells
摘要
Abstract
BACKGROUND: It is of great significance to explore the expression and effect of LINGO-1 in the differentiation of spinal cord derived neural stem cells (SpNSCs) for regulating neural stem cell differentiation and repairing spinal cord injury. OBJECTIVE: To investigate the expression features and biological effects of LINGO-1 in the differentiation of SpNSCs. METHODS: SpNSCs were isolated from the rat spinal cord and cultured in vitro. The expression characteristics of LINGO-1 was observed through double immunofluorescence staining of LINGO-1 and Nestin (neural stem cells), β-Tubulin III (neurons), GFAP (astrocytes) and O4 (oligodendrocyte) at 0-5 days of differentiation. SpNSCs isolated from the rat spinal cord were cultured in vitro and divided into siRNA group and control group. The siRNA group was transfected with LINGO-1 shRNA lentiviral vector to down-regulate the expression of LINGO-1, and the control group was transfected with Scramble-shRNA lentiviral vector. The growth of neurites was detected by immunofluorescence staining at 5 days after transfection.RESULTS AND CONCLUSION: The SpNSCs could differentiate into neurons, astrocytes and oligodendrocytes. LINGO-1 was expressed in SpNSCs, neurons and oligodendrocytes, but not in astrocytes. The neurite length of the siRNA group was significantly longer than that of the control group (P < 0.05). In summary, the SpNSCs have the potential of multi-directional differentiation, and LINGO-1 has a negative effect on the neurite growth.关键词
干细胞/神经干细胞/LINGO-1/脊髓神经干细胞/克隆球/突起/新疆维吾尔自治区自然科学基金分类
医药卫生引用本文复制引用
许刚,赵晨光,孙玮,琚芬,袁华,牟翔..LINGO-1在脊髓神经干细胞分化过程中的表达特征及效应[J].中国组织工程研究,2017,21(33):5394-5399,6.基金项目
新疆维吾尔自治区自然科学基金(2016D01C395) the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2016D01C395 （2016D01C395）
