西南国防医药2017，Vol.27Issue(11)：1145-1147,3.DOI:10.3969/j.issn.1004-0188.2017.11.001
芍药苷预处理对微血管内皮细胞缺氧复氧模型的干预作用
Intervention effects of pretreatment with paeoniflorin on microvascular endothelial cell hypoxia-reoxygenation model
摘要
Abstract
Objective To explore the intervention effects of pretreatment with paeoniflorin on microvascular endothelial cell hypoxia-reoxygenation model.Methods Microvascular endothelial cell hypoxia-reoxygenation models of rats were prepared and randomly divided into five groups,i.e.a control group (blank control),a hypoxia-reoxygenation group (treated with ischemiareoxygenation),and three paeoniflorin pretreatment groups (treated with ischemia-reoxygenation +low,medium and high doses of paeoniflorin for pretreatment).Flow cytometry (FCM) was used to detect the cell apoptosis in each group,and ELISA method was applied to detecting the expression of TNFα and IL-1β.Results Compared with the hypoxia-reoxygenation group,the levels of TNFα and IL-1β in the three dosage groups of paeoniflorin pretreatment were significantly lower (P ＜ 0.01),but there was no significant statistical difference among the three groups (P ＞ 0.05);the detection results of FCM snowed that the apoptosis index in the paeoniflorin pretreatment groups was significantly lower than that in the hypoxia-reoxygenation group (P ＜ 0.05),but there was no significant statistical difference among the three groups,either (P ＞ 0.05).Conclusion Pretreatment with paeoniflorin can reduce the apoptosis of microvascular endothelial cell in rats induced by ischemia-reoxygenation and protect microvascular endothelial cell.This may be correlated to the reduction of the expression of TNFα and IL-1β.关键词
芍药苷/微血管/内皮细胞/细胞凋亡/缺氧-复氧Key words
paeoniflorin/microvascular/endothelial cell/cell apoptosis/ischemia-reoxygenation分类
医药卫生引用本文复制引用
赵伟,高宇勤,郝霁萍,贺少辉,马宏恩..芍药苷预处理对微血管内皮细胞缺氧复氧模型的干预作用[J].西南国防医药,2017,27(11):1145-1147,3.基金项目
国家自然科学基金面上项目(81673787) （81673787）
西安市科技资助项目(SF1416) （SF1416）
