云南农业大学学报（自然科学）2017，Vol.32Issue(5)：780-786,7.DOI:10.16211/j.issn.1004-390X(n).2017.05.005
马铃薯纺锤块茎类病毒云南分离物的序列分析
Sequence Analysis of Potato Spindle Tuber Viroid Isolates in Yunnan
摘要
Abstract
[Purpose] Our aim was to understand the molecular variation and pathogenic mechanism of potato spindle tuber viroid (PSTVd) of Yunnan Province.[Method] The suspected tubers of PSTVd were collected from two different areas in Yunnan.Total RNA was extracted and reverse transcripted to cDNA.RT-PCR detection was conducted using PSTVd specific primers,and then the am plified products were purified,cloned and sequenced.[Results] The results showed that the full length fragments of PSTVd are 357 bp,which named as PSTVd-DasongpingYN (KX159281) and PSTVd-LiangheYN (KX159282),respectively.The phylogenetic trees were constructed with two PSTVd isolates from Yunnan and other PSTVd strains reported worldwide.Meanwhile,the sequence comparison and the second structure prediction were analyzed among PSTVd-DasongpingYN,PSTVdLiangheYN and different pathogenic PSTVd strains (mild,medium and severe),indicating that the PSTVd-DasongpingYN and PSTVd-LiangheYN were more close to the mild PSTVd strains.[Conclusion] The similarity of PSTVd-DasongpingYN and PSTVd-LiangheYN was 97.5 ％.There are 9 different bases mainly concentrated in the variable region (V) and the right terminal region (TR) which implied that there are obvious differences in two PSTVd Yunnan isolates.PSTVd-DasongpingYN and PSTVd-LiangheYN belong to the mild strain of PSTVd.关键词
马铃薯纺锤块茎类病毒/反转录-聚合酶链式反应/序列分析/二级结构预测Key words
potato spindle tuber viroid/reverse transcription polymerase chain reaction (RT-PCR)/sequence analysis/second structure prediction分类
农业科技引用本文复制引用
孙爱丽,王柱华,高锡春,谭涵月,杨艳丽,蔡红,朱有勇..马铃薯纺锤块茎类病毒云南分离物的序列分析[J].云南农业大学学报（自然科学）,2017,32(5):780-786,7.基金项目
云南省高校科技创新团队支持计划(云科教[2014] 22号) （云科教[2014] 22号）
云南省农业产业化专项项目(云财农[2013] 364号) （云财农[2013] 364号）
云南省科技创新人才计划项目(2011HB026). （2011HB026）
