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玛咖根腐病菌的生物学特性及防治药剂室内筛选

魏蜜 朱洁倩 张伟 金文闻 傅本重 李国元

云南农业大学学报(自然科学)2017,Vol.32Issue(5):787-792,6.
云南农业大学学报(自然科学)2017,Vol.32Issue(5):787-792,6.DOI:10.16211/j.issn.1004-390X(n).2017.05.006

玛咖根腐病菌的生物学特性及防治药剂室内筛选

Biological Characteristics of Pathogen Causing Maca Root Rot Disease and Its Fungicides Laboratory Screening

魏蜜 1朱洁倩 1张伟 2金文闻 1傅本重 2李国元3

作者信息

  • 1. 湖北工程学院,生命科学技术学院/特色果蔬质量安全控制湖北省重点实验室,湖北孝感432000
  • 2. 湖北大学生命科学学院,湖北武汉430062
  • 3. 华中科技大学生命科学与技术学院,资源生物学与生物技术研究所,湖北武汉430074
  • 折叠

摘要

Abstract

[Purpose] Our aim was to elucidate the biological characteristics and suitable fungicides for the pathogen of root rot on maca and to provide theoretical basis for high yield and high quality cul tivation of maca.[Method] The mycelium growth of F.avenaceum ROT-2 were studied through single factor test method under different conditions including different culture medium,different carbon source,nitrogen source,temperature,pH,lighting and ventilation conditions.In addition,the fungicides against the pathogen were investigated in this study.[Results] The results indicated that the pathogen mycelium grew well on PDA and OA under 21 ℃,the colony diameter was larger and the mycelium was thicker.Starch and NaNO3 were the best carbon and nitrogen resource,respectively.The pathogen hyphe were grown well under pH 4-10,while the optimum pH for mycelial growth was 6,and the lethal temperature was 55 ℃.The dark and stuffy conditions were more conducive to the mycelium growth.[Conclusion] The most effective fungicide was 80% Mancozeb among the three tested reagents,and the relative inhibition rate was 88.8% under the mass concentration of 1.00 mg/ mL,when the EC50 values was 0.140 9 mg/mL.

关键词

玛咖/根腐病/燕麦镰刀菌/生物学特性/药剂筛选

Key words

Lepidium meyenii/root rot/Fusarium avenaceum/biological characteristics/fungicides screening

分类

农业科技

引用本文复制引用

魏蜜,朱洁倩,张伟,金文闻,傅本重,李国元..玛咖根腐病菌的生物学特性及防治药剂室内筛选[J].云南农业大学学报(自然科学),2017,32(5):787-792,6.

基金项目

湖北省自然科学基金青年项目(2016CFB204) (2016CFB204)

国家自然科学基金资助项目(31600055) (31600055)

特色果蔬质量安全控制湖北省重点实验室开放基金重点资助项目(2016K07). (2016K07)

云南农业大学学报(自然科学)

OA北大核心CSCDCSTPCD

1004-390X

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