云南农业大学学报(自然科学)2017,Vol.32Issue(5):787-792,6.DOI:10.16211/j.issn.1004-390X(n).2017.05.006
玛咖根腐病菌的生物学特性及防治药剂室内筛选
Biological Characteristics of Pathogen Causing Maca Root Rot Disease and Its Fungicides Laboratory Screening
摘要
Abstract
[Purpose] Our aim was to elucidate the biological characteristics and suitable fungicides for the pathogen of root rot on maca and to provide theoretical basis for high yield and high quality cul tivation of maca.[Method] The mycelium growth of F.avenaceum ROT-2 were studied through single factor test method under different conditions including different culture medium,different carbon source,nitrogen source,temperature,pH,lighting and ventilation conditions.In addition,the fungicides against the pathogen were investigated in this study.[Results] The results indicated that the pathogen mycelium grew well on PDA and OA under 21 ℃,the colony diameter was larger and the mycelium was thicker.Starch and NaNO3 were the best carbon and nitrogen resource,respectively.The pathogen hyphe were grown well under pH 4-10,while the optimum pH for mycelial growth was 6,and the lethal temperature was 55 ℃.The dark and stuffy conditions were more conducive to the mycelium growth.[Conclusion] The most effective fungicide was 80% Mancozeb among the three tested reagents,and the relative inhibition rate was 88.8% under the mass concentration of 1.00 mg/ mL,when the EC50 values was 0.140 9 mg/mL.关键词
玛咖/根腐病/燕麦镰刀菌/生物学特性/药剂筛选Key words
Lepidium meyenii/root rot/Fusarium avenaceum/biological characteristics/fungicides screening分类
农业科技引用本文复制引用
魏蜜,朱洁倩,张伟,金文闻,傅本重,李国元..玛咖根腐病菌的生物学特性及防治药剂室内筛选[J].云南农业大学学报(自然科学),2017,32(5):787-792,6.基金项目
湖北省自然科学基金青年项目(2016CFB204) (2016CFB204)
国家自然科学基金资助项目(31600055) (31600055)
特色果蔬质量安全控制湖北省重点实验室开放基金重点资助项目(2016K07). (2016K07)