云南农业大学学报（自然科学）2017，Vol.32Issue(5)：805-810,6.DOI:10.16211/j.issn.1004-390X(n).2017.05.008
红景天内生放线菌拮抗菌株的筛选及抗药性标记
Screening of an Antagonistic Endophytic Actinomycete from Rhodiola rosea and its Resistance to Fungicide
摘要
Abstract
[Purpose] In order to explore new potential biocontrol member for controlling plant disease,the endophytic actinomycetes from Rhodiola rosea with fungistasis were researched.[Method] In this study endophytic actinomycetes from the joint position of root and stem of Rhodiola rosea were isolated and screened the antagonistic activity to plant pathogens using confrontation culture method and mycelial growth rate method.Then,the 16S rRNA gene sequence and antifungal spectrum of the strain with the best fungistasis were also determined.And to assess the possibility the strain used with fungicide,the resistance of the strain with the best fungistasis to commonly-used fungicides were tested.[Results] The result showed that 7 isolates possessed the antagonistic activity among 19 endophytic actinomycetes,and the fungistasis of Hj3 (Streptomyces variabilis) was the strongest and could inhibit all plant pathogens provided by the experiment.Also,Hj3 could grow well above the highly dose of boscalid,polyoxin,nalidixic acid,and ampicillin,respectively.[Conclusion] There is certain advantage for Hj3 to control usual plant disease.关键词
红景天内生放线菌/拮抗/抑菌谱/抗药性Key words
endophytic actinomycete from Rhodiola rosea/antagonistic/antifungal spectrum/resistance分类
农业科技引用本文复制引用
史晓晶,王铁霖,于春艳,高磊..红景天内生放线菌拮抗菌株的筛选及抗药性标记[J].云南农业大学学报（自然科学）,2017,32(5):805-810,6.基金项目
忻州师范学院博士科研启动项目 （）
忻州师范学院科研基金资助项目(201716) （201716）
中国中医科学院院级自主选题项目(ZZ2015027). （ZZ2015027）
