云南农业大学学报（自然科学）2017，Vol.32Issue(5)：818-822,5.DOI:10.16211/j.issn.1004-390X(n).2017.05.010
家蚕抗菌肽基因BmCecropinB6的克隆及重组蛋白的自诱导表达
Cloning of BmCecropinB6 Antibacterial Peptide Gene and Auto-induction Expressing of Recombinant Proteins from Bombyx mori
摘要
Abstract
[Purpose] Bombyx mori CecropinB6 (BmCecropinB6) is an important antibacterial peptide of silkworm immune system,it has strong antibacterial function on against gram positive and gram negative bacteria,but auto-induction expressing of recombinant protein has been reported.[Method] In this study,the total RNA as a template was extracted from the silkworm midgut tissue,a pairs of specific primer was designed,then B.CecropinB6 (BmCecropinB6) cDNA fragment was cloned by RT-PCR technology.pET32a-BmCecropinB6 prokaryotic expression vector was constructed,BmCecropinB6 recombinant protein was expressed by auto-induction expressing system,and expressing yields was further analyzed.[Result] The BmCecropinB6 gene is 192 bp nucleotides in sizes,and encodes 63 amino acids,the auto-induction expressing of recombinant BmCecropinB6 protein is about 27 ku,the expressing yields is 41％.[Conclusion] The research establish a basis for further application of BmCecropinB6 antimicrobial peptides.关键词
家蚕/抗菌肽/自诱导/BmCecropinB6Key words
silkworm/antibacterial peptide/auto-induction/BmCecropinB6分类
医药卫生引用本文复制引用
王靖,王丽仙,朱灵明,尹建华,叶青霞,孙艳,谢昆..家蚕抗菌肽基因BmCecropinB6的克隆及重组蛋白的自诱导表达[J].云南农业大学学报（自然科学）,2017,32(5):818-822,5.基金项目
云南省大学生创新创业训练计划项目(DCXM163018) （DCXM163018）
红河学院大学生科技创新项目(SZ1520) （SZ1520）
红河学院应用型科学研究项目(XJY15207) （XJY15207）
红河学院博士专项项目(XJ15B13) （XJ15B13）
云南省昆虫生物医药研发重点实验室开放课题. （）
