中国输血杂志2017，Vol.30Issue(9)：992-994,3.DOI:10.13303/j.cjbt.issn.1004-549x.2017.09.006
ABO新生儿溶血病放散试验异常结果原因的体外模拟实验探讨
In vitro simulations for abnormal result analysis in elution test
摘要
Abstract
Objective To analyze the cause of inverted results in the neonatal hemolytic disease elution test tor type A (or B) newborn infants.Methods Group O Plasma were acquired from pregnant females and were then processed by 2-Me (2 mL plasma with 2 mL 2-Me),followed by incubation with 2 ml of group A (or B) erythrocytes in 37℃ for 1 h.Later the erythrocytes were extracted from the mixture and an additional cycle were performed.The plasma before and after erythrocytes treatment were measured for IgG anti-A and anti-B antibody titer.The extracted erythrocytes went through the elution test at 56℃ and the elution liquid were tested for anti-A and anti-B tests.Results After the two cycles of type A erythrocyte absorption,the anti-A titers dropped to 0 and anti-B dropped 1-4 titers while,the anti-B titers dropped to 0 and anti-A dropped to 1-2 titers for plasma processed by type B erythrocyte;After Ac and Bc were absorbed for the first time,anti-A and anti-B were both detected.However,the second time after absorption,the Ac eluate only detected anti-B and Bc eluate only detected anti-A.Conclusion The A (or B) antibody which were eluted from B (or A) group neonatal were,in fact,low titer anti-AB.关键词
ABO血型/新生儿溶血病/放散试验/低效价抗体Key words
ABO blood group/heamolytic disease of the newborn/eluation test/lower-titer antibodies分类
临床医学引用本文复制引用
马春娅,杨璐,付丽辉,张晓娟,于洋,陈麟凤,汪德清..ABO新生儿溶血病放散试验异常结果原因的体外模拟实验探讨[J].中国输血杂志,2017,30(9):992-994,3.基金项目
“十三五”全军后勤科研项重点项目子课题(BWS16J006) （BWS16J006）
