中国比较医学杂志2017，Vol.27Issue(11)：19-23,5.DOI:10.3969.j.issn.1671-7856.2017.11.005
CKLF1-C19对昆明小鼠的急性毒性研究
Exploration of acute toxicity effect of CKLF1-C19 on KM mice
摘要
Abstract
Objective To explore the acute toxicity effect of CKLF1-C19,a polypeptide of chemokine-like factor 1(CKLF1),on the KM mice. Methods A total of 40 KM mice,half male and half female,were randomly divided into 2 groups. The mice in the experimental group were injected with CKLF1-C19 at a dose of 25 mL/kg(100 μg/mL,1 mg/mL and 10 mg/mL)through the tail vein,and those in the control group received an equal volume of sterile saline solution. Changes in the body weight of the mice were recorded the day after treatment, and the general conditions of mice in the experimental group were observed closely and compared with the normal group. Then blood samples were taken from the abdominal aorta to measure liver and kidney function. Tissue samples of liver, kidney, spleen and lung were taken for histopathological examination by HE staining. Results In the maximum tolerance test,the mice of the two groups were in good condition, and their body weight was increased gradually, without significant difference between the experimental group and the control group(P > 0.05). There was no death within 14 days. The blood biochemical indexes of liver and kidney function showed no significant differences between the two groups(P > 0.05). The gross appearances of heart, liver,kidney,spleen and lung were normal in the two mouse groups, and the pathological examination with HE staining showed normal clear structure with no obvious changes in these organs of each group. Conclusions Our results demonstrate that CKLF1-C19 has no acute toxicity effect on mice.关键词
CKLF1/急性毒性/小鼠Key words
CKLF1/Acute toxicity/Mice分类
医药卫生引用本文复制引用
朱琦,陈涛,刘蓉,陈国勤,孙红,陈晓雯,龙航,石燕情,谭亚夏..CKLF1-C19对昆明小鼠的急性毒性研究[J].中国比较医学杂志,2017,27(11):19-23,5.基金项目
广州呼吸疾病国家重点实验室开放课题项目(编号:2014SKLRD-O04). （编号:2014SKLRD-O04）
