中国比较医学杂志2017，Vol.27Issue(11)：38-43,6.DOI:10.3969.j.issn.1671-7856.2017.11.008
抑制作用及机制研究芍药苷对增殖性瘢痕成纤维细胞增殖
Inhibitory effect and mechanism of paeoniflorin on proliferation of hypertrophic scar fibroblasts
摘要
Abstract
Objective To explore the inhibitory effect and related mechanism of paeoniflorin on proliferation of hypertrophic scar(HS)fibroblasts. Methods HS fibroblasts were cultured with 0(control),200,400,800 μmol/L of paeoniflorin for 24,48,and 72 h. Cell viability was detected by MTT assay. Cell apoptosis was detected using Hoechst staining. Cell cycle was measured by flow cytometry. The levels of type I collagen(COL I)and type III collagen(COL III)were detected by enzyme linked immunosorbent assay(ELISA). The expressions of transforming growth factor-β (TGF-β)/Smad signaling pathway related proteins, as well as matrix metalloproteinase 1(MMP1)and MMP13 were detected by Western blot. Results 200, 400, 800 μmol/L of paeoniflorin reduced the cell viability of HS fibroblasts significantly(P < 0.01), with the nuclei turning pale and shrunken, and caused cell cycle arrest at G1 phase(P <0.01). Moreover, the levels of COL I and COL III in the cells were decreased significantly(P < 0.01), and the expressions of MMP1, MMP13, TGF-β1, p-Smad2 and p-Smad3 were down-regulated significantly(P < 0.01). Conclusions Paeoniflorin can obviously inhibit the proliferation and collagen synthesis of hypertrophic scar fibroblasts,probably through inhibition of the TGF-β1/Smad signaling pathway.关键词
芍药苷/增生性瘢痕/成纤维细胞/增殖/胶原合成Key words
Paeoniflorin/Hypertrophic scar/HS/Fibroblasts/Proliferation/Collagen synthesis分类
医药卫生引用本文复制引用
王雷,马园园,李亚玲,周粤闽..抑制作用及机制研究芍药苷对增殖性瘢痕成纤维细胞增殖[J].中国比较医学杂志,2017,27(11):38-43,6.基金项目
河南省省部共建课题项目:自体细胞培养复合型人工真皮的构建及临床应用研究(编号:201401014). （编号:201401014）
