中国比较医学杂志2017，Vol.27Issue(11)：44-49,6.DOI:10.3969.j.issn.1671-7856.2017.11.009
IL-37 b通过抑制树突状细胞相关的免疫应答缓解感染性休克
IL-37b alleviates septic shock through inhibition of immune responses related to dendritic cells
摘要
Abstract
Objective To express the recombinant IL-37b protein and to investigate its role in the regulation of immune responses. Methods The prokaryotic expression vector pET28/IL-37b was constructed. The recombinant IL-37b protein was induced to be expressed and was purified using Ni2+-NTA gel column. C57BL/6 J mice were treated with IL-37b and injected with lipopolysaccharide(LPS)to induce septic shock,and the expression levels of IL-1β,IL-6,IFN-γ in the serum of the mice were detected. Dendritic cells from bone marrow of the mice were isolated and cultured, and were treated with IL-37b. After LPS-induced activation, the expression levels of marker molecules such as CD40, CD80 and MHCII on the cell surface, and cytokines such as IL-1β, IL-10, IL-12 and TNF-α in the culture supernatants were detected by flow cytometry. The CD4 +T cells from mice were isolated and the inhibitory effects of IL-37b on the expression of IFN-γ,TNF-α and IL-10 in the culture supernatants of T cells after induction by LPS were detected. Results IL-37b reduced the expression levels of proinflammatory cytokines in the serum of septic shock mice. IL-37b also inhibited the expression of co-stimulatory molecules and proinflammatory cytokines of the mouse dendritic cells, and suppress the activation of CD4 +T cells in vitro. Conclusions Purified recombinant IL-37b protein has high bioactivity, and can alleviate septic shock in organisms through inhibiting the activation of dendritic cells and related T cell immune responses.关键词
IL-37b/纯化/树突状细胞/免疫应答/感染性休克Key words
IL-37b/Purification/Dendritic cells/Immune responses/Septic shock分类
医药卫生引用本文复制引用
李梦媛,燕正强,韦荣飞,杨星九,朱瑞敏,高苒..IL-37 b通过抑制树突状细胞相关的免疫应答缓解感染性休克[J].中国比较医学杂志,2017,27(11):44-49,6.基金项目
中国医学科学院医学与健康科技创新工程-重大协同创新项目-协同创新团队资助(编号:2016-I2M-3-019). （编号:2016-I2M-3-019）
