中国现代医学杂志2017，Vol.27Issue(28)：1-5,5.DOI:10.3969/j.issn.1005-8982.2017.28.001
慢性应激对大鼠肾脏水通道蛋白1的影响及机制研究
Effect of chronic stress on renal aquaporin-1 expression in rats and its mechanism
摘要
Abstract
Objective To investigate the effect of chronic stress (induced by foot shock) on renal aquaporin-1 (AQP1) expression in rats and its mechanism. Methods The chronic stress model was established in male Sprague-Dawley (SD) rats by foot shock. The rats were divided into control group, foot shock group, renal sympathetic nerve denervation group, renal sympathetic nerve denervation plus foot shock group, Captopril plus foot shock group and Tempol plus foot shock group (6 in each group). Tail-cuff method was applied to measure blood pressure of the rats. RT-PCR was applied to detect the AQP1 mRNA level in the kidney tissues. Immunohistochemistry was used to observe the expression and distribution of AQP1 in rat kidneys. Results Compared to the control group, the expression of AQp1 and the blood pressure markedly increased in the foot shock group (P < 0.05), the expression of AQP1 in the kidney tissues was decreased in the renal sympathetic nerve denervation group (P < 0.05) without significant increase of blood pressure. Compared to the foot shock group, the expression of AQP1 was decreased in the renal sympathetic nerve denervation plus foot shock group, the Captopril plus foot shock group and the Tempol plus foot shock group accompanied by reduction of blood pressure (P < 0.05). Conclusions Renal AQP1 expression in rats is regulated by sympathetic nerves. Foot shock causes the increase of AQP1 expression, which is due to the increased renal sympatheic activity. In addition, oxidative stress and renin-agiontensin system regulate the expression of AQP1. AQP1 may play a certain role in the regulation of hypertension induced by chronic stress.关键词
水通道蛋白1/应激性高血压/氧化应激/肾交感神经Key words
AQP1/stress-induced hypertension/oxidative stress/renal sympathetic nerve system分类
医药卫生引用本文复制引用
朱叶,卫郅星,陈晓帆,张国兴..慢性应激对大鼠肾脏水通道蛋白1的影响及机制研究[J].中国现代医学杂志,2017,27(28):1-5,5.基金项目
国家自然科学基金(No:81270316,81470563) （No:81270316,81470563）
国家级大学生创新训练项目(No:2015suda047) （No:2015suda047）
