中国现代医学杂志2017，Vol.27Issue(28)：6-11,6.DOI:10.3969/j.issn.1005-8982.2017.28.002
远志汤有效成分对APP/PS1小鼠氧化损伤的保护作用机制研究
Study on protective effect of effective components of Polygala Decoction on oxidative damage in APP/PS1 double transgenic mice
摘要
Abstract
Objective To observe the regulative effect of β-asarone and Tenuigenin on the pI3K/Akt/GSK-3β signaling pathway in App/pS1 double transgenic mice, to explore the mechanism of polygala Decoction in prevention and treatment of Alzheimer's disease. Methods Three-month-old App/pS1 double transgenic mice were divided into model group, Donepezil hydrochloride group (0.33 mg/kg·d), high-dose β-asarone and Tenuigenin group, medium-does β-asarone and Tenuigenin group and low-dose β-asarone and Tenuigenin group (18.5, 37.0 and 74.0 mg/kg·d). C57BL/6 mice of the same age were selected as the control group. Morris water maze test and new object recongnition task were used to measure the spatial learning and memory ability. Spectrophotometer was used to detect the activity of SOD, catalase (CAT) and glutathione peroxidase (GSh-pX) and the content of MDA. Western blot was performed to evaluate the expressions of glycogen synthase kinase-3β (GSK-3β), p-GSK-3β, Akt and p-Akt. Results β-asarone and Tenuigenin significantly ameliorated the cognitive defect of App/pS1 double transgenic mice, increased the activity of SOD, CAT and GSh-pX, while decreased the content of MDA, suppressed the activation of GSK-3β and enhanced the activation of Akt. Conclusions β-asarone and Tenuigenin can inhibit the oxidative stress of brain tissue, and exhibit positive therapeutic effect on Alzheimer's disease by regulating the activity of the pI3K/Akt/GSK-3β signaling pathway.关键词
β-细辛醚/远志皂苷/APP/PS1双转基因小鼠/PI3K/Akt/GSK-3β信号通路Key words
β-asarone/Tenuigenin/APP/PS1 double transgenic mouse/PI3K/Akt/GSK-3β signaling pathway分类
医药卫生引用本文复制引用
董海影,丛欢,王俊苹,弓箭,柏青杨..远志汤有效成分对APP/PS1小鼠氧化损伤的保护作用机制研究[J].中国现代医学杂志,2017,27(28):6-11,6.基金项目
国家自然科学基金青年项目(No:81403131) （No:81403131）
