中国现代医学杂志2017，Vol.27Issue(28)：12-15,4.DOI:10.3969/j.issn.1005-8982.2017.28.003
野生型PTEN过表达对体外活化肝星状细胞内钙离子浓度的影响
Effect of wild-type PTEN over-expression on intracellular calcium ion concentration in activated hepatic stellate cells in vitro
摘要
Abstract
Objective To investigate the effect of over-expression of wild-type phosphatase and tensin homology deleted on chromosome ten (PTEN) on the intracellular calcium ion concentration in activated hepatic stellate cells (hSCs) in vitro. Methods Using adenoviral vector, wild-type PTEN gene was transduced into activated rat hSC (hSC-T6) in vitro, and the expressions of PTEN mRNA and protein were detected by qRT-pCR and Western blot. Then with the help of laser scanning confocal microscope (LSCM), the changes of intracellular calcium ion concentration in hSCs were detected using calcium ion fluorescence probe Rhod-2/AM. The cells were grouped as follows: control group, in which the viral medium was replaced by DMEM when hSCs were transfected with adenovirus; Ad-GFp group, in which hSCs were transfected with adenovirus expressing green fluorescent protein (GFp) alone; and Ad-pTEN group, in which hSCs were transfected with adenovirus harboring both wild-type PTEN and GFP genes. Results The wild-type PTEN was successfully transduced into activated hSCs by adenoviral vector. The intracellular calcium ion concentration of the hSCs in the Ad-pTEN group significantly decreased compared with the control group and the Ad-GFp group (P < 0.05). however, no significant difference was observed in the intracellular calcium ion concentration of hSCs between the control group and the Ad-GFp group (P > 0.05). Conclusions The over-expression of wild-type PTEN can significantly reduce the intracellular calcium ion concentration in activated hepatic stellate cells in vitro.关键词
肝星状细胞/第10号染色体缺失的磷酸酶张力蛋白同源物基因/细胞内钙离子浓度Key words
hepatic stellate cell/PTEN/intracellular calcium ion concentration分类
医药卫生引用本文复制引用
郝礼森,宋小杰,王玉兰,刘玉龄,宋洁,张明婷,靳丽敏,张朋垒..野生型PTEN过表达对体外活化肝星状细胞内钙离子浓度的影响[J].中国现代医学杂志,2017,27(28):12-15,4.基金项目
河北省自然科学基金(No:H2013209327) （No:H2013209327）
中国肝炎防治基金会天晴肝病研究基金资助项目(No:CFHPC20132078) （No:CFHPC20132078）
