中国畜牧杂志2017，Vol.53Issue(11)：30-34,5.DOI:10.19556/j.0258-7033.2017-11-030
西藏牛Y染色体USP9Y基因与mtDNA D-loop区遗传多态性研究
Genetic Diversity of Y Chromosome USP9Y Gene and Mitochondrial DNA D-loop Region in Tibetan Cattle
摘要
Abstract
This study is to investigate the genetic diversity and the origins of Y chromosome and mitochondrial DNA D-loop region in Tibetan cattle.Using PCR amplification and restriction enzyme digestion methods to detect the polymorphism of Y chromosome USP9Y gene of 30 Tibetan cattle;the complete D-loop region sequences of mitochondrial DNA from 30 individuals in Tibetan cattle were determined by PCR amplification and sequencing methods.Bioinformatics method was used to analyze the genetic polymorphism of 44 D-loop sequences in Tibetan cattle (of which 14 sequences of Tibetan were obtained from GenBank).30 individuals of Tibetan cattle were analyzed using a recently discovered USP9Y marker that could distinguish taurine from zebu more efficiently.The results showed that the USP9Y sequence-tagged sites of Tibetan cattle were detected two DNA fragments,471 bp and 552bp,and the 552 bp DNA fragment can't be digested by SspI enzyme,indicating that Tibetan cattle only had Y1 and Y2 haplogroups.The haplogroup frequencies of Y1 and Y2 were 0.067 and 0.933,respectively.To compare with 44 individuals of the complete mitochondrial DNA D-loop region in Tibetan cattle,8 individuals were proved to belong to yak which detected in this study,indicating the introgression of genes from the yak.62 mutation sites and 21 haplotypes were found in 36 individuals of complete mitochondrial DNA D-loop sequences of Tibetan cattle.Nucleotide diversity was 0.0064 and haplotype diversity was 0.9560,suggesting an abundant mitochondrial genetic diversity in Tibetan cattle.Phylogenetic analysis showed that the maternal origins of Tibetan cattle were from Bos taurus and Bos indicus,and the taurine haplotypes dominated the Tibetan cattle population (95.24％).Our results demonstrated that Tibetan cattle have rather abundant mitochondrial genetic diversity,consisting of two taurine paternal haplogroups.The Tibetan cattle should be classified as Bos taurus,and our study also revealed a female yak and zebu introgression in Tibetan cattle.关键词
西藏牛/Y染色体/USP9Y/mtDNA D-loop/遗传多态性/起源Key words
Tibetan cattle/Y chromosome/USP9Y/Mitochondrial DNA D-loop/Genetic diversity/Origins分类
农业科技引用本文复制引用
夏小婷,马志杰,张成福,蓝贤勇,陈宏,雷初朝..西藏牛Y染色体USP9Y基因与mtDNA D-loop区遗传多态性研究[J].中国畜牧杂志,2017,53(11):30-34,5.基金项目
国家肉牛牦牛产业技术体系资助(CARS-37) （CARS-37）
