中国药房2017，Vol.28Issue(33)：4695-4698,4.DOI:10.6039/j.issn.1001-0408.2017.33.24
毛黄堇药材的质量标准研究
Study on Quality Standard of Corydalis tomentella
摘要
Abstract
OBJECTIVE:To establish the quality standard of Corydalis tomentella. METHODS:The medicinal material was identified in respects of property,microscopic characteristics and TLC. The contents of moisture,total ash and water soluble ex-tract were determined. The content of dehydrocavidine was determined by HPLC. The determination was performed on Agilent C18 column with mobile phase consisted of acetonitrile-phosphate buffer solution(containing 20 mmol/L monopotassium phosphate,10 mmol/L diethylamine,0.1% phosphoric acid)(28:72,V/V)at the flow rate of 1.0 mL/min. The detection wavelength was set at 347 nm,and the column temperature was 35 ℃. The sample size was 10 μL. RESULTS:The original plant is perennial herbs. The me-dicinal material often shrank into a ball. The main root was in conical shape;the rhizomes and roots were distinctly chapped;leaves curled and broken,and flowers were yellowish-white. The pollen grains were round-like in shape;square and columnar crys-tals were found;there were a large number of nonglandular hairs. The bordered pit,threaded and reticulate catheter were found;wood fiber was also found. TLC spots were clear and well-separated. The content of moisture were 7.5%-18.5%,total ash were 20.5%-26.2%,and extract were 29.9%-46.4%. The linear range of dehydrocavidine were 0.04008-2.4048 μg(r=0.9999);RSDs of precision,stability and reproducibility tests were lower than 2.0%. The recoveries were 95.6%-102.5%(RSD=2.3%,n=9). CONCLUSIONS:The established standard can be used for quality evaluation of C. tomentella.关键词
毛黄堇/质量标准/薄层色谱法/高效液相色谱法/脱氢卡维丁Key words
Corydalis tomentella/Quality standard/TLC/HPLC/Dehydrocavidine分类
医药卫生引用本文复制引用
吴佳,胡娟娟,张德伟..毛黄堇药材的质量标准研究[J].中国药房,2017,28(33):4695-4698,4.基金项目
国家科技重大专项课题(No.2014ZX09304307) （No.2014ZX09304307）
