中国药房2017，Vol.28Issue(33)：4699-4702,4.DOI:10.6039/j.issn.1001-0408.2017.33.25
藏药水柏枝的质量标准研究
Study on Quality Standard of Tibetan Medicine Myricaria germanica
摘要
Abstract
OBJECTIVE:To establish the quality standard of Tibetan medicine Myricaria germanica. METHODS:M. germani-ca was identified in respects of properties,microscopic characteristics and TLC. The contents of moisture,ash and extract were de-termined. HPLC method was adopted for content determination of gallic acid. The determination was performed on Diamonsil C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution(5:95,V/V)at the flow rate of 1.0 mL/min. The detection wavlengths were set at 270 nm. The column temperature was 30 ℃ and the sample size was 10 μL. RESULTS:M. ger-manica was cylindrical in shape,and there were many narrow strip alternate leaflets,which were brittle,easily broken,weak in smell,mild in flavor. Cork cell surface and lower epidermal cell surface showed polygon;most of xylon were bunched;there were many clusters of calcium oxalate. TLC spots were clear and well-separated. The contents of moisture,total ash,acid-insoluble ash, water-soluble extract and ethanol were 6.83%-8.12%,4.01%-5.01%,1.06%-1.98%,17.91%-22.65%,11.29%-15.51%. The linear range of gallic acid were 3.13-50 μg/mL(r=0.9997);RSDs of precision,stability and reproducibility tests were lower than 1.0%;the recoveries were 94.0%-100.8%(RSD=2.27%,n=9). CONCLUSIONS:Established standard can be used for quality evalua-tion of Tibetan medicine M. germanica.关键词
藏药/水柏枝/质量标准/没食子酸/薄层色谱法/高效液相色谱法Key words
Tibetan medicine/Myricaria germanica/Quality standard/Gallic acid/TLC/HPLC分类
医药卫生引用本文复制引用
多杰,马志良,多杰拉旦,尼玛才让,东知多杰,赛桑杰..藏药水柏枝的质量标准研究[J].中国药房,2017,28(33):4699-4702,4.基金项目
青海省自然科学基金面上项目(No.2015-ZJ-910) （No.2015-ZJ-910）
青海省藏药新药开发重点实验室项目(No.2017-ZJ-Y15) （No.2017-ZJ-Y15）
