中国药房2017，Vol.28Issue(33)：4714-4717,4.DOI:10.6039/j.issn.1001-0408.2017.33.29
火把花根片中间体的HPLC指纹图谱研究
Study on HPLC Fingerprints of Huobahuagen Tablets Intermediate
摘要
Abstract
OBJECTIVE:To establish the HPLC fingerprints for Huobahuagen tablets intermediate. METHODS:HPLC was per-formed on Inertsil ODS-4 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid (gradient elution) at the flow rate of 0.75 mL/min. The detection wavelength was set at 220 nm,and column temperature was 30 ℃. The sample size was 10 μL. Using wilforgine as reference,HPLC chromatograms of 10 batches of samples were determined. Common peak identification and similarity evaluation were performed by using Similarity Evaluation System for TCM Chromatographic Fingerprint (2004 A edi-tion). RESULTS:There were 25 common peaks in HPLC chromatograms of 10 batches of samples,and similarity degrees were higher than 0.9. After validated,HPLC chromatograms of 10 batches of samples were in good agreement with control fingerprints. CONCLUSIONS:The established fingerprint can provide reference for identification and quality evaluation of Huobahuagen tab-lets intermediate.关键词
火把花根片/中间体/高效液相色谱法/指纹图谱Key words
Huobahuagen tablets/Intermediate/HPLC/Fingerprint分类
医药卫生引用本文复制引用
郑丁丁,张小梅,杨怡,陈一龙,励娜,姚媛媛,杨大坚..火把花根片中间体的HPLC指纹图谱研究[J].中国药房,2017,28(33):4714-4717,4.基金项目
重庆市应用开发计划项目(No.cstc2014yykfC10005) （No.cstc2014yykfC10005）
