中国药房2017，Vol.28Issue(33)：4722-4724,3.DOI:10.6039/j.issn.1001-0408.2017.33.31
HPLC法测定安神补脑液中防腐剂的含量
Content Determination of Antiseptics in Anshen Bunao Oral Liquid by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the determination of antiseptics in Anshen bunao oral liquid. METHODS:HPLC method was adopted. The determination was performed on Agilent Zorbax SB-C18 column with mobile phase consisted of ace-tonitrile-0.05% phosphorice acid solution (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 236 nm,and column temperature was 30℃. The injection volume was 10μL. RESULTS:The linear range of benzoic acid and eth-ylparaben were 12.85-128.54 μg/mL(r=0.9999)and 4.05-40.48 μg/mL(r=0.9998),respectively. The limits of quantitation were 0.514,1.012 μg/mL. The limits of detection were 0.171,0.353 μg/mL. RSDs of precision,stability and reproducibility tests were all lower than 1.0%. The recoveries were 99.38%-100.21%(RSD=0.31%,n=6),97.96%-100.72%(RSD=0.91%,n=6). CON-CLUSIONS:The method is simple and accurate,and can be used for content determination of antiseptics in Anshen bunao oral liq-uid.关键词
高效液相色谱法/安神补脑液/防腐剂/含量Key words
HPLC/Anshen bunao oral liquid/Antiseptics/Content分类
医药卫生引用本文复制引用
陈灵君,王发英,王伟影..HPLC法测定安神补脑液中防腐剂的含量[J].中国药房,2017,28(33):4722-4724,3.基金项目
浙江省公共科技条件平台项目(No.2012E60008) （No.2012E60008）
