摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of ferulic acid,catalpol,emodin and emodin methylether in Yishen yansi pills. METHODS:Double wavelength HPLC method was adopted. The separation was performed on Kromasil-C18 column with mobile phase methanol-0.1% phosphate solution(78:22,V/V)at flow rate of 1.0 mL/min. The detection wavelengths were set at 210 nm for catalpol,280 nm for ferulic acid,emodin and emodin methylether. The column temperature was 30 ℃,and sample size was 10 μL. RESULTS:The linear ranges were 0.0908-2.2700 μg/mL for ferulic acid(r=0.9999), 0.1990-4.9750 μg/mL for catalpol (r=0.9993),0.3540-8.8500 μg/mL for emodin (r=0.9999),0.3360~8.4000 μg/mL for emodin methylether(r=0.9997),respectively. The limits of quantitation were 14.66,8.75,10.26,13.68 ng,and the limits of de-tection were 5.66,2.67,3.78,4.25 ng,respectively. RSDs of precision,stability and reproducibility tests were lower than 2.0%. The recoveries were 101.15%-102.73%(RSD=0.58%,n=6),99.20%-101.69%(RSD=1.20%,n=6),100.90%-101.36%(RSD=0.16%,n=6),95.64%-98.96%(RSD=1.49%,n=6),respectively. CONCLUSIONS:The method is accurate,reliable,simple and suitable for simultaneous determination of 4 kinds of components in Yishen yansi pills.关键词
高效液相色谱法/双波长/益肾衍嗣丸/阿魏酸/梓醇/大黄素/大黄素甲醚/含量Key words
HPLC/Double wavelength/Yishen yansi pills/Ferulic acid/Catalpol/Emodin/Emodin methylether/Content分类
医药卫生
