摘要
Abstract
Objective To investigate the clinical effect of mesenchymal stem cells induced by bortezomib in the treatment of osteoporosis in rats. Methods Totally 75 healthy female rats were selected to establish the ovariectomized osteoporosis model of rats and randomly divided into 5 groups. The osteocalcin levels of the 5 groups were measured. Another 75 healthy experimental rats were selected to obtain bone marrow mesenchymal stem cells, which were cultured in vitro until form a colony. The bone marrow mesenchymal stem cells were injected into the bone marrow cavity of the rats in the 5 groups, rats in group 1-5 were given 0, 0. 1, 0. 3, 0. 5, 1. 5 mg/kg bortezomib for 4 weeks, the peripheral blood of rats was extracted daily and osteocalcin levels were measured. After 4 weeks, the rats were killed, and the long bones of the rats were taken for sections, the bone mineral density, proliferation index and apoptosis index of bone marrow mesenchymal stem cells were measured. Results There was no significant difference in osteocalcin levels between the 5 groups before treatment ( P > 0. 05 ) , after treatment, the osteocalcin levels of the 5 groups were from high to low as follows:group 1, group 2, group 3, group 4, group 5. The bone density of the long bone and the proliferation indexes of bone and marrow mesenchymal stem cells in group 5 were significantly higher than those in the other 4 groups ( P < 0. 05 ) , and the apoptotic index in group 5 was lower than those in the other 4 groups ( P < 0. 05 ) . Conclusion Mesenchymal stem cells induced by bortezomib is effective in the treatment of ovariectomized osteoporosis in rats, and it is feasible in the clinical treatment of postmenopausal osteoporosis.关键词
硼替佐米/间充质干细胞/骨质疏松/增殖指数/凋亡指数/骨钙素水平/骨密度Key words
bortezomib/mesenchymal stem cell/osteoporosis/proliferation index/apoptosis index/osteocalcin level/bone density分类
医药卫生
