中国药业2017，Vol.26Issue(23)：17-19,3.DOI:10.3969/j.issn.1006-4931.2017.23.005
固相萃取-反相高效液相色谱法检测木瓜丸中双酯型生物碱限度
Determination of Diester Alkaloid Limit in Mugua Pills by SPE-HPLC
摘要
Abstract
Objective To establish an SEP-HPLC method for simultaneous determination of three kinds of diester alkaloids in Mugua Pills. Methods The Eclipse XDB-C18 column ( 250 mm × 4. 6 mm, 5 μm ) was adopted, the mobile phase was 0. 1% ammonium acetate solution ( pH was adjusted to 3. 5 by glacial acetic acid ) - acetonitrile ( 75 :25 ) , the flow rate was 1. 0 mL/min, the detection wave-length was 235 nm, and the column temperature was 40 ℃. Results The concentrations of new aconitine, aconitine and hypaconitine were in the range of 1. 25-12. 5 μg/mL, 3. 10-31. 0 μg/mL, 3. 24-32. 4 μg/mL, the average recovery was 98. 01% and RSD was 1. 50% ( n=6 ) . Conclusion The method is rapid, simple, accurate, reliable and sensitive. It can be used to determine the contents of three toxic diester alkaloids in Mugua Pills and to control the quality of the drugs.关键词
木瓜丸/双酯型生物碱/固相萃取/高效液相色谱法Key words
Mugua Pill/diester alkaloids/solid phase extraction/HPLC分类
医药卫生引用本文复制引用
巩晓宇,陆燕萍,陈喜悦,邱凤邹,陈秋燕..固相萃取-反相高效液相色谱法检测木瓜丸中双酯型生物碱限度[J].中国药业,2017,26(23):17-19,3.基金项目
广东省深圳市龙岗区科技发展资金医疗卫生(扶持类)项目[ YLWS20150514153303078 ]. （扶持类）
