摘要
Abstract
Objective To establish an HPLC method for simultaneous determination of baicalin, aloeemodin, rhein, glycirrizic acid, emodin and chrysophanol in Jinzhen Oral Solution. Methods The chromatographic column was Phenomenex C18 column(250 mm × 4. 6 mm, 5 μm), the mobile phase was methanol ( A ) -0. 05% phosphoric acid solution ( B ) with gradient elution, the flow rate was 1. 0 mL/min, the column temperature was 30 ℃, the detection wavelength was 280 nm for baicalin ( 0-15. 0 min ) , 254 nm for aloeemodin and rhein ( 15. 0-35. 0 min ) , 237 nm for glycirrizic acid ( 35. 0-39. 8 min ) , 254 nm for emodin and chrysophanol ( 39. 8-50. 0 min ) . Results The linear range of baicalin, aloeemodin, rhein, glycirrizic acid, emodin and chrysophanol were 0. 0432-2. 1625 μg, 0. 0128-0. 6380 μg, 0. 0185-0. 9260 μg, 0. 0358-1. 7925 μg, 0. 0498-2. 4880 μg, 0. 0529-2. 6435 μg, respectively. The average recovery rates were 98. 84%, 99. 49%, 99. 89%, 100. 31%, 99. 55%, 99. 54%, respectively, the RSDs were 1. 10%, 1. 03%, 1. 49%, 1. 23%, 0. 98%, 1. 19%( n=9 ) . Conclusion This method is simple and fast, which can be used for quality control of Jinzhen Oral Solution.关键词
高效液相色谱法/金振口服液/黄芩苷/芦荟大黄素/大黄酸/甘草酸/大黄素/大黄酚Key words
HPLC/Jinzhen Oral Solution/baicalin/aloeemodin/rhein/glycirrizic acid/emodin/chrysophanol分类
医药卫生
