中国卒中杂志2017，Vol.12Issue(11)：1000-1004,5.DOI:10.3969/j.issn.1673-5765.2017.11.006
基于基因表达谱筛查小鼠脑缺血再灌注后星形胶质细胞活化相关基因
Gene Microarray Analysis of Astrocytes in Acute Ischemic Stroke Mice Based on Gene Expression Omnibus
摘要
Abstract
Objective To explore the signal pathway which played a key role in astrocytes following cerebral ischemia/reperfusion by microarray, and to analyze the key genes which might involve in the activation process of astrocytes. Methods Data of 8 chips of astrocytes from mouse brain of Affymetrix Gene Chip Mouse Genome 430 2.0 arrays were collected from Gene Expression Omnibus (GEO) database. Eight mice were divided into two groups: Experimental group (Ischemia with 60 min MCAO plus 24 h reperfusion, n=4)and Sham operation group(n=4).The differentially expressed genes were detected,then the signal pathways were analyzed to screen the key signal pathway and analyze the genes which played an important role in regulation. Results A total of 1966 genes were determined as differential genes in astrocytes from acute ischemia/reperfusion mice, among which, 1210 genes expression were up regulated and 756 genes were down regulated. Pathway analysis determined 154 differential pathways. Further analysis found that four pathway including mitogen-activated protein kinases (MAPK) pathway, apoptosis, cell cycle and p53 pathway, which played an important role in activation process of astrocytes following ischemia/reperfusion.Moreover,Flnc1 and Ccnd1 were key genes in activation process of astrocytes in response to ischemia/reperfusion. Conclusion Acute cerebral ischemia/reperfusion resulted in abundant differential expressed genes that played important roles in activation process of astrocytes. Through analyzing the differential genes, the new targets which potentially play the important regulation role in ischemia/reperfusion injury could be screened.关键词
脑缺血再灌注/基因芯片/差异表达/信号通路Key words
Cerebral ischemia/reperfusion/Gene microarray/Differential expression/Signal pathway引用本文复制引用
董雯,王拥军..基于基因表达谱筛查小鼠脑缺血再灌注后星形胶质细胞活化相关基因[J].中国卒中杂志,2017,12(11):1000-1004,5.基金项目
国家重点研发计划(2017YFC1307501)首都医科大学附属北京天坛医院青年科研基金项目(No.2016-YQN-19)国家自然科学基金(81701138) （2017YFC1307501）
