中华耳科学杂志2017，Vol.15Issue(5)：546-551,6.DOI:10.3969/j.issn.1672-2922.2017.009.
AIFM1基因突变对Harlequin小鼠年龄相关耳蜗听觉功能的影响
The Effect of Apoptosis Inducing Factor Gene on Peripheral Hearing Function During Aging
摘要
Abstract
Objective To study the functional role of apoptosis inducing factor gene (AIF) in cochlear homeosta-sis during aging. Methods Harlequin mice (2 and 6 months old) that were lack of the AIFM1 gene were used. Com-pound action potentials (CAP) and cochlear microphonics (CM) elicited by tone-bursts were recorded from the round window. The level of cochlear outer hair cell damage was evaluated using phalloidin staining. Results In the 2-months age group, the average compound action potentials threshold was 18.6 ± 1.6 dB SPL for wild-type (WT) mice (n=6) and 22.22.2 dB SPL for knockout (KO) mice (n=6), respectively (Student's t-test, P>0.05). However, for the 6 months age group, the average CAP threshold was 25.70 ± 1.69 dB SPL for WT mice (n=5), which was significantly lower than that for KO mice (42.10 ± 3.59 dB SPL, n=5) (Student's t-test, t=4.129, P<0.01). Comparisons of CAP amplitudes be-tween the WT and KO mice revealed a significant difference at higher frequencies (30, 35 and 40 kHz;two-way ANO-VA,P<0.05), but not at middle and low frequencies. The CM amplitude I/O functions were non-linear in both WT and KO mice at both 2 months and 6 months of age, with no significant difference between the WT and KO mice (Two-way ANOVA, P>0.05). Consistent with this functional assessment, comparison of missing OHCs revealed a significant dif-ference at basal turn of cochlea between the WT and KO groups at the age of 6 months (Student t-test, t=15.11, P<0.0001). Conclusions Loss of AIFM1 gene function accelerates age-related outer hair cell degeneration and high fre-quency hearing loss.关键词
AIFM1/耳蜗电图/毛细胞/听力损失Key words
AIFM1/ECochG/Hair Cells/Hearing Loss分类
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熊彬彬,胡博华,赵晓明,刘钊,赵立东,孙伟..AIFM1基因突变对Harlequin小鼠年龄相关耳蜗听觉功能的影响[J].中华耳科学杂志,2017,15(5):546-551,6.基金项目
珠海市科技计划项目(20171009E030055)Science and Technology Planning Project of Zhuhai City (20171009E030055) （20171009E030055）
