中华耳科学杂志2017，Vol.15Issue(5)：555-560,6.DOI:10.3969/j.issn.1672-2922.2017.011.
EGB761对脉冲所致噪声性聋大鼠内耳形态及功能影响的研究
Effects of EGB761 on Inner Ear Mn-SOD Activity in Rats with Noise Induced Deafness
摘要
Abstract
Objective To investigate effects of EGB761 on ABR threshold, hair cell nucleus staining and manga-nese superoxide dismutase (Mn-SOD) activity in the inner ear in rats with by impulse noise induced deafness. Methods SD rats (n=80) were randomly divided into a blank control (n=20) and an experiment (n=60) group. Rats in the experi-ment group received impulse noise exposure (150 dB SPL, pulse width=30 ms, repetition rate=1/min, total=3), and were then randomly selected to be examined immediate, or to receive intraperitoneal injection of EGB761 (Ginaton 4 ml/kg) or equivalent volume of saline, bid for 7 consecutive days before being examined. Results ABR thresholds in the ex-periment group were significantly elevated following impulse noise exposure (P<0.01), regardless of timing or treat-ment (P>0.05). After 7 days, ABRs thresholds improved in both saline and EGB761 treated animals, but more so in those treated with EGB761 than in those treated with saline (P=0.02). Immunofluorescence showed normal arrangement of outer hair cells nuclei in rats without noise exposure. Immediately following noise exposure, punctate deletion of out-er hair cell nuclei was noticed in the basal turn. On Day 7, significant lamellar deletion of outer hair cell nuclei was re-corded in rats treated with saline, more severe than immediately following exposure and than in those treated with EGB761 whose loss of outer hair cells nuclei was sparse and scattered. Mn-SOD results showed no statistically signifi-cant difference between noise exposed and non-exposed animals immediately following exposure (P>0.05);whereas on Day 7, inner ear Mn-SOD activity in rats treated with normal saline was significantly lower than immediately following exposure (P<0.01) and then in EGB761 treated rats (P=0.01). Conclusions EGB761 appears to mitigate auditory func-tion decline, hair cell loss and oxidative stress reaction, and improve the prognosis in pulse noise induced deafness.关键词
噪声性聋/听性脑干反应/锰超氧化物歧化酶/银杏叶提取物Key words
Noise Induced Hearing Loss/Auditory Brainstem Response/Mn-SOD/EG761分类
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董颖婕,吴玮,王刚,韩浩伦,张弛,刘旭,屈昌北,刘钢..EGB761对脉冲所致噪声性聋大鼠内耳形态及功能影响的研究[J].中华耳科学杂志,2017,15(5):555-560,6.基金项目
全军"十二五"重大课题( AWS11J003) 资助 Supported by the Major project of 12 th Five-Year for PLA ( AWS11J003)Supported by the Major project of 12 th Five-Year for PLA (AWS11J003) （ AWS11J003）
