中华耳科学杂志2017，Vol.15Issue(5)：561-566,6.DOI:10.3969/j.issn.1672-2922.2017.012.
CTB增强PsaA疫苗对肺炎链球菌急性中耳炎的保护作用
Intranasal Immunization with PsaA/CTB Enhances Adaptive Immune Responses and Protection against Pneumococcal Acute Otitis Media
摘要
Abstract
Objective To study specific immune responses and protective effects against pneumococcal acute oti-tis media in BALB/c mice immunized intranasally with PsaA protein vaccine and cholera toxin B (CTB) as a mucosal adjuvant. Methods BALB/c mice aged 6 to 8 weeks old were randomly divided into 3 groups and were respectively im-munized intranasally with PsaA protein (PsaA group, 15 μg PsaA protein), PsaA protein and CTB immunization (PsaA/CTB group, 15μg PsaA/4μg CTB), or CTB (CTB group, 4μg CTB) at the same dose two times weekly for three weeks. Two weeks after the last immunization, nasal lavage fluid (NW), middle ear lavage fluid (MEL) and bronchoalveolar la-vage fluid (BALF) were collected for detection of specific IgA antibody. Serum was collected for detection of specific IgG, IgG1 and IgG2a antibodies. Mice were challenged with type 14 streptococcus pneumoniae by the tympanic mem-brane route. Middle ear inflammation was evaluated by histopathology 5 days after challenging. Results Compared to mice immunized with PsaA or CTB, anti-PsaA specific IgA in mucosal lavages were elevated significantly in mice im-munized with PsaA/CTB (P<0.05);and specific IgG, IgG1 and IgG2a antibody levels in serum with PsaA/CTB immuni-zation were also significantly higher (P<0.05). Significantly more inflammatory cells in the middle ear cavity were found in PsaA or CTB groups than in the PsaA/CTB group on day 5 after pneumococcal challenge (P<0.05). Conclu-sions Mucosal immunization with PsaA/CTB is efficacious in generating mucosal and systemic immune responses and in protecting against acute otitis media. CTB is an effective mucosal immune adjuvant for PsaA. The study provides an experimental basis for designinh a new generation of pneumococcal vaccine.关键词
霍乱毒素B/肺炎链球菌/黏膜免疫/PsaA/急性中耳炎Key words
Cholera toxin B/Streptocoocus pneumoniae/Mucosal immunity/PsaA/Acute otitis media分类
医药卫生引用本文复制引用
戴文佳,梁琼,刘向,李雯,徐江红..CTB增强PsaA疫苗对肺炎链球菌急性中耳炎的保护作用[J].中华耳科学杂志,2017,15(5):561-566,6.基金项目
National Natural Science Foundation (No. 81200736,No. 81000406) （No. 81200736,No. 81000406）
