中成药2017，Vol.39Issue(11)：2236-2242,7.DOI:10.3969/j.issn.1001-1528.2017.11.003
活血康脑颗粒对大鼠脑缺血再灌注损伤的保护作用
Protective effects of Huoxue Kangnao Granules on cerebral ischemia-reperfusion injury in rats
摘要
Abstract
AIM To explore the protective effects of Huoxue Kangnao Granules (Astragali Radix,Morindae officinalis Radix,Puerariae lobatae Radix,etc.) on cerebral ischemia-reperfusion injury in rats.METHODS The rat model for middle cerebral artery occlusion (MCAO) was established,SD rats were randomly divided into sham operation group,model group,positive control group,Huoxue Kangnao Granules low-,middle-and highdose groups.Nerve function score was tested.Volume of cerebral infarction was calculated by TTC staining.SOD,MDA,6-keto-PGF1α,TXB2,IL-1 β and TNF-α were detected by ELISA.The expression levels of MMP-9 and ICAM-1 were detected by RT-PCR.To observe the influence of Huoxue Kangnao Granules on platelet aggregative function,clotting time was analyzed by automatic blood coagulation analyzer,plasma T-PA and PAI activities were determined by ELISA,and hemorheology indices were detected by automatic biochemical analyzer.RESULTS Huoxue Kangnao Granules could significantly decrease cerebral infarction volume,improve nerve function,reduce MDA content,increase SOD activity;increase 6-keto-PGF1 α level,reduce TXB2 content;decrease TNF-α and IL-1β expressions;inhibit MMP-9 and ICAM-1 expressions;extend KPTT and PT;elevate t-PA activity,inhibit PAI activity;decrease whole blood viscosity,enhance erythrocyte deformation ability.Each dosage group of Huoxue Kangnao Granules had a positive correlation between dose and response.CONCLUSION Huoxue Kangnao Granules can protect cerebral ischemia-reperfusion injury in rats,whose mechanisms may be related to anti-free radical injury,anti-thrombus,anti-cerebral vasospasm,improving cerebrovascular permeability,anti-inflammatory,anti-coagulation and improving abnormal changes of hemorheology.关键词
活血康脑颗粒/脑缺血再灌注损伤/抗自由基/抗炎/抗凝血/抗血栓/血液流变学Key words
Huoxue Kangnao Granules/cerebral ischemia-reperfusion injury/anti-free radical/anti-inflammatory/anticoagulation/antithrombus/hemorheology分类
医药卫生引用本文复制引用
万嘉洋,万海同,何昱,虞立,杨洁红,周惠芬..活血康脑颗粒对大鼠脑缺血再灌注损伤的保护作用[J].中成药,2017,39(11):2236-2242,7.基金项目
国家自然科学基金资助项目(81630105,81603531) （81630105,81603531）
浙江省自然科学基金资助项目(LZ17H270001) （LZ17H270001）
浙江省中医药管理局资助项目(2016ZZ010) （2016ZZ010）
