中成药2017，Vol.39Issue(11)：2249-2254,6.DOI:10.3969/j.issn.1001-1528.2017.11.005
滋肾育胎丸对DOR大鼠内分泌的影响
Effects of Zishen Yutai Pills on endocrine in DOR rats
摘要
Abstract
AIM To study the effects of Zishen Yutai Pills (Codonopsis Radix,Dipsaci Radix,Morindae officinalis Radix,etc.) on endocrine in diminished ovarian reserve (DOR) rats.METHODS Female SD rats with normal oestrous cycle were induced by intragastric administration of 55 mg/(kg · d) tripterygium glycosides for fourteen days to establish DOR model,and then divided into Zishen Yutai Pills high-,low-dose groups,Bujiale group,model control group and negative control group.After 35 days' administration,serum estradiol (E2),follicle-stimulating hormone (FSH),luteinizing hormone (LH) and progesterone (P) levels were detected by ELISA.And ovarian tissue estrogen receptor (ER),follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) expressions were detected by immunohistochemical method.RESULTS Compared with the negative control group,there were significant changes in levels of E2,FSH,LH,P,and expressions of ER,FSHR,LHR in the model control group (P ＜ 0.05),but there were no statistical significances in body weight,ovary index,uterus index and serum FSH/LH level between the two groups (P ＞ 0.05).Compared with the model control group,E2 level was increased in the Zishen Yutai Pills high-,low-dose groups and Bujiale group,FSH,LH levels were decreased,and they also promoted ER expression and restrained FSHR,LHR excessive expressions,which were statistically significant (P ＜ 0.05).P level was significangly increased in different dose groups (P ＜ 0.05),FSH/LH in the high-dose group was obviously decreased (P ＜ 0.05),and body weight,ovary index,uterus index in various intervening groups showed no statistical significances (P ＞ 0.05).CONCLUSION Zishen Yutai Pills can improve ovarian reserve function in DOR rats by affecting the expression of ovarian tissue hormone receptor and regulating endocrine function.关键词
滋肾育胎丸/卵巢储备功能低下/内分泌/大鼠Key words
Zishen Yutai Pills/diminished ovarian reserve/endocrine/rats分类
医药卫生引用本文复制引用
樊耀华,陈思,李婧,赵颖,张芝桐,陈颖慧,黎子莹..滋肾育胎丸对DOR大鼠内分泌的影响[J].中成药,2017,39(11):2249-2254,6.基金项目
广州中医药大学大学生创新创业训练项目(201510572105) （201510572105）
广州白云山中一药业有限公司罗元恺滋肾育胎丸中青年科研基金项目(140210) （140210）
