中成药2017，Vol.39Issue(11)：2279-2284,6.DOI:10.3969/j.issn.1001-1528.2017.11.011
甘草黄酮纳米混悬剂的制备及其体外溶出率
Preparation of nanosuspensions of flavonoids from Glycyrrhizae Radix et Rhizoma and the in vitro dissolution rate
摘要
Abstract
AIM To prepare nanosuspensions of flavonoids from Glycyrrhizae Radix et Rhizoma and to determine the in vitro dissolution rate.METHODS Precipitation-high pressure homogenization method was adopted in the preparation of nanosuspensions.With mean particle size and polydispersity index (PDI) as evaluation indices,concentrations of flavonoids,povidone K30 (PVP K30) and polyethylene glycol 400 (PEG 400) as influencing factors,central composite design-response surface method was applied to optimizing the preparation.For the freedried powder prepared by freeze-drying method,the optimal kind and ratio of lyoprotectant were screened.Then the in vitro dissolution rates of freeze-dried powder and physical mixture were compared.RESULTS The optimal conditions were determined to be 10.00 mg/mL for flavonoids' concentration,and 2.30 mg/mL for both PVP K30 and PEG 400 concentrations,the mean particle size and PDI were (172.3 ± 1.2) nm and 0.175 ± 0.004,respectively.The optimal lyoprotectant was 5％ mannitol-lactose (3 ∶ 2),the mean particle size and PDI after redissolution were (239.7 ±2.1) nm and 0.193 ±0.032,respectively.The in vitro dissolution rate of lyoprotectant reached 87.7％ within 60 min,which was much higher than that of physical mixture (less than 30％).CONCLUSION Nanosuspension can effectively improve the in vitro dissolution rate of flavonoids from Glycyrrhizae Radix et Rhizoma.关键词
甘草黄酮/纳米混悬剂/体外溶出率/沉淀-高压均质法/星点设计-效应面法Key words
flavonoids from Glycyrrhizae Radix et Rhizoma/nanosuspensions/in vitro dissolution rate/precipitation-high pressure homogenization method/central composite design-response surface method分类
医药卫生引用本文复制引用
吴超群,李小芳,严敏嘉,孙强,赵甜甜,马祖兵..甘草黄酮纳米混悬剂的制备及其体外溶出率[J].中成药,2017,39(11):2279-2284,6.基金项目
四川省教育厅重点项目(15ZA0094) （15ZA0094）
