植物研究2017，Vol.37Issue(6)：870-875,6.DOI:10.7525/j.issn.1673-5102.2017.06.010
外源NO对转基因白桦外源基因表达及DNA甲基化的影响
Effects of Exogenous Nitric Oxide on Exogenous Gene Expression and DNA Methylation in Transgenic Birch
摘要
Abstract
In order to investigate the relationship between transgene expression and the methylation of genomic DNA induced by 2 mmol · L-1 SNP in Transgenic birch,the effect of SNP (sodium nitroprusside) on the expression of exogenous BGT,methyltransferase gene DRM,MET and the level of DNA methylation were determined in transgenic birch callus.The activities of defense enzymes and the MDA (malondialdehyde) content in transgenic birch were increased significantly,which indicated high concentration of NO had harm on the normal life of birch cells.The methyltransferase DRM and MET genes were up-regulated and the methylation level of genomic DNA was increased from 10.6％ to 16.5％.The transcription level of BGT was improved at 6 h and only 0.46 folds of the control at 3 d,which suggested the expression of exogenous BGT gene in transgenic brich was affected by high concentration of NO and the level of genomic methylation.The results revealed the response patterns of exogenous gene and methyltransferase gene and determined the genomic methylation level and physiological and biochemical characteristics to the high concentration of NO in birch,which will provide some references for further study of epigenetic regulation and regulation of exogenous gene expression in transgenic plants.关键词
NO/转基因白桦/基因表达/甲基化Key words
NO/transgenic birch/gene expression/methylation分类
农业科技引用本文复制引用
孙丰坤,李思达,李吉祥,陈晓慧,曾凡锁..外源NO对转基因白桦外源基因表达及DNA甲基化的影响[J].植物研究,2017,37(6):870-875,6.基金项目
中央高校基本科研业务费专项资金项目(2572014DA04) （2572014DA04）
国家自然科学基金项目(J1210053,31200463)The central university basic research business expenses special fund project(2572014DA04) （J1210053,31200463）
National Natural Science Foundation of China(J1210053,31200463) （J1210053,31200463）
