中国中西医结合杂志2017，Vol.37Issue(11)：1339-1344,6.DOI:10.7661/j.cjim.20171013.239
宁心痛颗粒含药血清及有效组分干预THP-1源性巨噬细胞泡沫化的效应与机制
Effect and Mechanism of the Foaming of THP-1 Derived Macrophages Intervened by NingXinTong Granules Containing Medicine Serum and Effective Components
摘要
Abstract
Objective To explore the effect and mechanism of the foaming of THP-1 derived macrophages intervened by NingXinTong Granules containing medicine serum and effective components.Methods THP-1 cells were induced by phorbol-1-myristate-13-acetate (PMA),differentiated into macrophages (Mψ),then divided into seven groups:blank group,that was macrophage group(Mψ group);model group,that was foam cell group(FC group);NingXinTong Granules containing medicine serum group(CMS group);blank control serum group(BCS group);astragalus polysaccharide group(APS group);Iigustilide group(LIG group);APS combining with LIG group(APS + LIG group).The foam cell formation rates were detected by high performance liquid chromatography (HPLC),and the expressions of SRA-Ⅰ,CD36,ABCA1,LXR-α and PPAR-γ were measured by Western blot technology.Results The levels of CE/ET and the protein expressions of SRA-Ⅰ,CD36,ABCA1,LXR-α and PPARγof the FC group were obviously higher than the Mψ group (P ＜0.05).The levels of CE/ET of the LIG,APS +LIG and CMS groups were significantly lower than the FC group (P ＜0.05).The protein expressions of SRA-Ⅰ and CD36 of APS,LIG,APS +LIG and CMS groups were remarkably lower than the FC group (P ＜0.05),and the protein expressions of ABCA1,LXR-α and PPAR-γ were noticeably higher than the FC group (P ＜0.05),except ABCA1 expression in APS group.The level of CE/ET of CMS group was markedly lower than the APS group (P ＜0.05).While the protein expression of ABCA1 of the CMS group was statistically higher than the APS group (P ＜0.05).Conclusion NingXinTong Granules containing medicine serum and effective components might inhibit the foaming of THP-1 derived macrophages,the mechanism of which might be regulating the balance of “intakereverse transport” cholesterol of the macrophage,that was down-regulating the expressions of SRA-Ⅰ and CD36,and up-regulating the expressions of ABCA1,LXR-α and PPAR-γ.关键词
宁心痛颗粒/含药血清/有效组分/THP-1源性巨噬细胞/泡沫细胞Key words
NingXinTong Granules/containing medicine serum/effective components/THP-1 derived macrophage/foam cell引用本文复制引用
何小丽,顾宁..宁心痛颗粒含药血清及有效组分干预THP-1源性巨噬细胞泡沫化的效应与机制[J].中国中西医结合杂志,2017,37(11):1339-1344,6.基金项目
国家自然科学基金面上项目(No.81173399) （No.81173399）
江苏省研究生科研创新基金项目(No.CXZZ11-0759) （No.CXZZ11-0759）
江苏省基础研究计划面上项目(江苏省自然科学基金)(No.BK20161115) （江苏省自然科学基金）
南京市十三五医学科技创新平台重大项目(No.ZDX16009) （No.ZDX16009）
