中药材2017，Vol.40Issue(5)：1020-1025,6.DOI:10.13863/j.issn1001-4454.2017.05.003
木香根腐病病原菌鉴定及生物学特性研究
The Primary Studies on Pathogen Identification and Biological Characteristics of Saussurea lappa
摘要
Abstract
Objective:To isolate and identify pathogen of Saussurea lappa root-rot disesse,to study the effects of culture,carbon,nitrogen,temperature,pH on the sporulation and hyphal growth of pathogens,and to research the primary biological features of the fungi to offer a theoretical foundation for the prevention of this disease in the field.Methods:By observing the morphological characters of pathogens and rDNA-ITS sequencing,searching similar sequences by Blast in Genbank,the molecular phylogenetic tree was constructed by MEGA 6.0 program in ITS sequence,and the pathogen 15b was identified as Fusarium oxysporum f.sp.ciceris isolate.Results:Biological characteristics measurement showed that PDA was the optimal medium for mycelium growth,the optimum carbon and nitrogen sources were soluble starch and peptone,the optimal temperature was 25 ℃,the optimal pH value was 6.The optimum carbon and nitrogen sources of sporulation were soluble starch and peptone,the optimal temperature was 30 ℃,and the optimal pH value was 7.The light had no obvious effect to mycelium,while the dark culture conditions in 24 h were suitable for the spore production,and mycelial lethal temperature was 61 ℃ for 10 min.Conclusion:Fusarium oxysporum f.sp.ciceris isolate is reported as the pathogen of root-rot disesse in Saussurea lappa for the first time.关键词
木香/根腐病/病原菌鉴定/生物学特性Key words
Saussurea lappa Decne./Root-rot disesse/Pathogen identification/Biological characteristics分类
医药卫生引用本文复制引用
韩凤,李巧玲,余中莲,林茂祥,杨成前,韩如刚,刘杰..木香根腐病病原菌鉴定及生物学特性研究[J].中药材,2017,40(5):1020-1025,6.基金项目
重庆市卫生局中医药科技项目(KJ20130161) （KJ20130161）
重庆市南川区科委项目(cx201509) （cx201509）
重庆市科委基本科研业务费项目(2016cstc-jbky-01304) （2016cstc-jbky-01304）
